Anti-Cytokeratin 5 Monoclonal Antibody for WB, IHC-P, ELISA - P13647

Core Product Specifications and Parameters

Biological Background: Cytokeratin 5 Function and Localization

• Keratin 5 (KRT5), also known as 58 kDa cytokeratin or CK-5, is a type II intermediate filament protein and a key member of the keratin family.
• Together with its obligate type I partner keratin 14 (KRT14), KRT5 forms the intermediate filament network in basal keratinocytes, providing mechanical stability and resilience to the basal layer of the epidermis. Related references: PMID:1372711, PMID:1694855
• KRT5 is highly expressed in the cytoplasm of basal keratinocytes in stratified epithelia such as skin and cornea. Related references: PMID:26758872, PMID:20128788, PMID:31302245
• KRT5 participates in the regulation of Langerhans cell recruitment to the epidermis, potentially by modulating macrophage chemotactic cytokines and CTNND1 localization (By similarity).
• Post-translational modifications include phosphorylation; the protein features a coiled-coil domain characteristic of intermediate filament assembly.
• Mutations in the KRT5 gene are associated with epidermolysis bullosa simplex (EBS), underscoring its essential role in epidermal integrity.
• The full-length protein has a predicted molecular weight of approximately 62 kDa and is also referred to as 58 kDa cytokeratin, CK-5, and type-II keratin Kb5.

Experimental Guidance and Technical Tips

• The monoclonal antibody was raised against a synthetic peptide corresponding to amino acids 491-590 of human KRT5. This C-terminal region is predicted to be exposed in the folded keratin filament. For confirmation, consider antigen-blocking experiments or use of KRT5 knockout/knockdown controls.
• For Western blotting, the expected molecular weight is approximately 62 kDa. Use appropriate loading controls and validate antibody specificity with cell lines known to express KRT5 (e.g., HaCaT, A431) and negative controls.
• This antibody is suitable for immunohistochemistry on paraffin-embedded tissues (IHC-P). Optimize antigen retrieval and primary antibody dilution for your specific sample type.
• For immunofluorescence, expect cytoplasmic filamentous staining. Use appropriate fixation methods to preserve intermediate filament structure.
• ELISA applications may require optimization of coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop supplier of IVD raw materials, providing high-quality antibodies like this anti-Cytokeratin 5 monoclonal antibody to support epithelial biology and dermatology research. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing solutions. Partner with CamelBio to streamline your assay development from concept to clinic.