Anti-Cytokeratin 5 (KRT5) Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P13647

Core Product Specifications and Parameters

Biological Background: Cytokeratin 5 Function and Localization

• Keratin, type II cytoskeletal 5 (KRT5), also designated Cytokeratin-5 (CK-5) or 58 kDa cytokeratin, is a type II keratin encoded by the KRT5 gene in humans.
• As a structural component of intermediate filaments, KRT5 partners with its obligate type I keratin 14 (KRT14) to form a resilient cytoskeletal network that provides mechanical integrity to basal keratinocytes of stratified epithelia. Related references: PMID:1372711, PMID:1694855
• KRT5 modulates immune cell recruitment by regulating the abundance of macrophage chemotactic cytokines and CTNND1 localization in keratinocytes, thereby influencing Langerhans cell homing to the epidermis.
• This keratin localizes to the cytoplasm, where it assembles into intermediate filaments.
• Expression is predominantly observed in keratinocytes and the corneal epithelium, as confirmed by protein-level detection.
• KRT5 is a phosphoprotein with a coiled-coil domain structure, and genetic variants are linked to epidermolysis bullosa simplex.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-100) of human KRT5, which may recognize full-length protein; validate in the relevant sample system.
• For Western blot, the predicted molecular weight is approximately 62 kDa; note that phosphorylation or other modifications may cause migration shifts.
• Suitable for immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC); consider optimization of antigen retrieval and antibody dilution for each tissue type.
• Cross-reactivity with mouse allows for rodent model studies; however, sequence alignment is advised to confirm epitope conservation.
• For immunoprecipitation (IP) and ELISA, the polyclonal nature may offer high sensitivity; confirm performance under your specific assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive one-stop sourcing partner for IVD raw materials, offering this anti-Cytokeratin 5 antibody alongside validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support your diagnostic and research workflows in epithelial biology. From initial concept to clinic, we provide seamless access to rare and standard targets, ensuring a reliable supply chain for your assay development needs.