Anti-Cytokeratin 4 (KRT4) Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P19013

Core Product Specifications and Parameters

Biological Background: Cytokeratin 4 (KRT4) Function and Localization

• Encoded by the KRT4 gene, also known as CK-4, K4, and type-II keratin Kb4.
• Core structural component of intermediate filaments in mucosal stratified squamous epithelia, providing mechanical stability and integrity.
• Forms obligate heteropolymers with the type I keratin KRT13, assembling into keratin intermediate filament networks.
• Predominantly expressed in the suprabasal layers of stratified epithelia of the esophagus, exocervix, vagina, mouth, and lingual mucosa; lower levels in basal cells.
• Localizes to the cytoplasm as part of the intermediate filament cytoskeleton, contributing to the structural framework of epithelial cells.
• Subject to methylation and implicated in disease variants.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100) of human KRT4, which may provide specificity for the full-length protein.
• For Western blot (WB), use appropriate extraction methods for intermediate filaments and verify the band near 56 kDa.
• For immunofluorescence (IF-P) and immunohistochemistry (IHC-P), consider fixation and permeabilization protocols suitable for cytoskeletal targets.
• Cross-reactivity with human and mouse is indicated; validate in the relevant species and tissue types, particularly mucosal epithelia.
• ELISA applications may require optimization of coating and blocking conditions for peptide antigens.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw materials, offering a comprehensive portfolio of validated antibodies, antibody pairs, and bulk ancillary reagents for cytokeratin and epithelial biology research. From monoclonal antibodies like this anti-KRT4 clone to rare target sourcing and technical consulting, we support diagnostic manufacturers from concept to clinic with reliable supply and expertise. Contact us to discuss your project's specific raw material needs.