Anti-Cytokeratin 2e (KRT2) Rabbit Polyclonal Antibody for WB and ELISA - P35908

Core Product Specifications and Parameters

Biological Background: Cytokeratin 2e (KRT2) Function and Localization

• KRT2, also known as CK-2e, K2e, or keratin-2 epidermis, is a type II cytokeratin encoded by the KRT2 gene in humans, serving as a structural component of intermediate filaments in suprabasal keratinocytes of stratified epidermis.
• It forms heteropolymers with type I keratins like KRT10, assembling into keratin intermediate filament networks that provide mechanical integrity to differentiating keratinocytes.
  Related references:
  PMID:12598329, PMID:1380918
• KRT2 contributes to terminal differentiation and cornification of keratinocytes and is crucial for establishing the epidermal barrier.
  Related references:
  PMID:9804344
• The protein is classified under the keratin family of intermediate filament proteins, featuring a coiled-coil domain structure typical of keratins.
• Subcellularly, KRT2 localizes to the cytoplasm, where it integrates into the cytoskeletal network.
• Post-translational modifications include phosphorylation and methylation, as indicated by proteomics and reference proteome annotations; it is also associated with disease variants linked to ichthyosis.
• Tissue specificity: Expressed in upper spinous and granular suprabasal layers of normal adult epidermis from most body sites (thigh, breast, foot sole, etc.), but absent in foreskin, squamous metaplasias, and carcinomas. Induced in certain benign keratoses and oral dysplasia with orthokeratinization.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to amino acids 1-220 of human KRT2 (NP_000414.2), targeting the N-terminal region of the protein.
• For Western blotting (WB), choose an appropriate positive control such as lysates from human epidermal tissues or cultured keratinocytes expressing KRT2; the predicted molecular weight is approximately 65 kDa.
• ELISA applications may require pairing with a suitable detection antibody; consider optimizing coating antigen and antibody concentrations for your assay format.
• As a polyclonal antibody, lot-to-lot variability can occur; validate each new batch in your specific experimental system before use.
• Cross-reactivity with mouse and rat KRT2 suggests potential use in rodent models, but verify species-specific performance in your application.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated one-stop IVD raw-material supplier, CamelBio connects diagnostic manufacturers and research laboratories with critical reagents like this anti-KRT2 polyclonal antibody, supporting investigations into epidermal biology, keratin intermediate filaments, and skin-related disorders. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets to streamline your workflow from concept to clinic. Contact our team to discuss your specific requirements and explore our comprehensive portfolio.