Anti-Cytokeratin 2e (KRT2) Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P35908

Core Product Specifications and Parameters

Biological Background: Cytokeratin 2e Function and Localization

• Keratin, type II cytoskeletal 2 epidermal (KRT2), also known as cytokeratin-2e (CK-2e), is a type II keratin encoded by the KRT2 gene, with a molecular weight of approximately 65 kDa.
• KRT2 is a structural component of intermediate filaments in suprabasal keratinocytes of the stratified epidermis, providing mechanical integrity and resilience to the differentiating epidermis (PubMed:12598329, PubMed:1380918). Related references: PMID:12598329 PMID:1380918
• It forms heteropolymers with type I keratins, such as KRT10, assembling into keratin intermediate filament networks essential for epidermal differentiation (PubMed:12598329). Related references: PMID:12598329
• Contributes to terminal differentiation and cornification of keratinocytes, playing a role in establishing the epidermal barrier (PubMed:9804344). Related references: PMID:9804344
• Expressed specifically in the upper spinous and granular suprabasal layers of normal adult epidermis from most body sites, but absent in foreskin and certain pathological tissues.
• Localizes to the cytoplasm, consistent with its role as an intermediate filament protein.
• Post-translational modifications include methylation and phosphorylation; associated with the disease ichthyosis.
• Keywords: Coiled coil, Cytoplasm, Disease variant, Ichthyosis, Intermediate filament, Keratin, Methylation, Phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a recombinant fusion protein covering amino acids 293–422 of human KRT2, a region within the rod domain; consider epitope specificity in your experimental design.
• This antibody is validated for WB, IF-P, IHC-P, and ELISA. For WB, the predicted band is around 65 kDa; validate in relevant tissue or cell lysates expressing KRT2.
• For immunohistochemistry, antigen retrieval and titration should be optimized in your sample system.
• Note that KRT2 expression is restricted to suprabasal layers; positive control tissues should include skin from body sites such as thigh or foot sole.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories worldwide. For projects focused on epidermal biology and keratinocyte differentiation, we offer validated antibody pairs, optimized monoclonal antibodies like this anti-KRT2 clone, and bulk ancillary reagents to streamline your assay development. Our team also assists in sourcing rare target raw materials, ensuring reliable supply continuity from concept to clinic.