Anti-Cytokeratin 19 (KRT19) Rabbit Monoclonal Antibody for IF, IHC, FC, ELISA - P08727

Core Product Specifications and Parameters

Biological Background: Cytokeratin 19 (KRT19) Function and Localization

• Keratin, type I cytoskeletal 19 (KRT19), also known as Cytokeratin-19 (CK-19) or Keratin-19 (K19), is a type I keratin intermediate filament protein.
• KRT19 is involved in the organization of myofibers by linking the contractile apparatus to dystrophin at the costameres of striated muscle, functioning together with KRT8.
• This protein is a member of the keratin gene family and forms heterodimers with type II keratins to assemble intermediate filaments.
• KRT19 is expressed in a wide range of epithelial tissues, including basal keratinocytes of hair follicles, sweat glands, mammary ducts, bile ducts, gastrointestinal tract, bladder urothelium, and oral/esophageal epithelia.
• At the subcellular level, it localizes to cytoplasmic intermediate filaments and specifically accumulates at costameres in muscle fibers, associating with dystrophin and spectrin.
• Key post-translational modifications include phosphorylation and methylation, and it contains coiled-coil domains critical for filament formation.
• KRT19 is also implicated in host-virus interactions and serves as a reference proteome member, with documented 3D structural data.

Experimental Guidance and Technical Tips

• The antibody was generated against a synthetic peptide from the C-terminal region (aa 301-400) of human KRT19; consider that this may affect recognition of isoforms or truncated forms. For western blotting, the predicted molecular weight is ~44 kDa, though actual migration may vary due to modifications.
• For immunofluorescence (IF/ICC, IF-P) and immunohistochemistry (IHC-P), validate in the relevant sample system, as KRT19 expression patterns are tissue-specific and may be influenced by fixation and antigen retrieval conditions.
• In flow cytometry (intracellular staining), optimize permeabilization protocols to ensure antibody access to the cytoplasmic intermediate filament network.
• Cross-reactivity with mouse and rat is reported; however, sequence alignment of the immunogen region across species should be verified if quantitative applications are needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to validated IVD raw materials, technical services, and consulting. From optimized monoclonal antibodies like anti-KRT19 to ancillary reagents and rare target sourcing, we support your workflow from discovery to clinical application. Contact us for bulk antibody pairs, custom formulations, and consulting tailored to epithelial biology and cytoskeleton research.