Anti-Cytokeratin 19 (KRT19) Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P08727

Core Product Specifications and Parameters

Biological Background: Cytokeratin 19 (KRT19) Function and Localization

• KRT19 encodes keratin, type I cytoskeletal 19 (also known as CK-19 or K19), a member of the type I keratin family of intermediate filament proteins.
• It plays a critical role in myofiber organization by partnering with KRT8 to link the contractile apparatus to dystrophin at the costameres of striated muscle.
• The protein is expressed in a wide range of simple and stratified epithelial tissues, including basal keratinocytes of hair follicles, sweat and mammary gland epithelia, bile ducts, gastrointestinal tract, bladder urothelium, and ectocervical epithelium.
• Subcellularly, KRT19 localizes to cytoplasmic intermediate filament networks and, in muscle fibers, accumulates at costameres—specialized regions of the sarcolemma that contain dystrophin and spectrin.
• Post-translational modifications include phosphorylation and methylation, and the protein features a central coiled-coil rod domain typical of keratins.
• Expression is also observed in vascular wall cells of umbilical cord veins and arteries, suggesting roles beyond epithelial tissues.
• UniProt lists this as a reference proteome member with a 3D structure determined, underscoring its well-characterized nature.

Experimental Guidance and Technical Tips

• The recombinant immunogen (aa 210–340) targets a region conserved in human, mouse, and rat KRT19, supporting the reported species cross-reactivity.
• For Western blot, a band near 44 kDa is expected; use epithelial cell lysates (e.g., HeLa, MCF-7) and verify with molecular weight markers.
• In IHC-P, optimize antigen retrieval (e.g., citrate buffer, pH 6.0) and test on known KRT19-positive tissues such as skin, colon, or breast carcinoma.
• For IF/ICC, fixation (e.g., 4% PFA) and permeabilization (e.g., 0.1% Triton X-100) conditions should be titrated to preserve the intermediate filament network.
• When performing ELISA, consider using recombinant KRT19 protein or peptide to establish standard curves and validate specificity.
• Always include appropriate controls: omit primary antibody, use isotype controls, and test on KRT19-negative cells/tissues (e.g., mesenchymal cells) to confirm signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers, labs, and research institutes from concept to clinic. This anti-Cytokeratin 19 polyclonal antibody enables precise detection for epithelial marker studies, cancer diagnostics, and tissue profiling. We offer validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare-target raw materials to accelerate your assay development.