Anti-Cytokeratin 19 (KRT19) Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P08727

Core Product Specifications and Parameters

Biological Background: Cytokeratin 19 (KRT19) Function and Localization

• Cytokeratin 19 (KRT19) is a type I keratin (acidic) that belongs to the intermediate filament family, playing a structural role in epithelial cells.
• KRT19 is a member of the keratin gene family, and its expression is often used as an epithelial cell marker.
• The protein is involved in the organization of myofibers; together with keratin 8 (KRT8), it helps link the contractile apparatus to dystrophin at the costameres of striated muscle.
• In non-muscle tissues, KRT19 is expressed in a wide range of simple and stratified epithelia, including basal keratinocytes of the skin, hair follicles, sweat glands, mammary gland ducts, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium.
• Subcellularly, KRT19 localizes to cytoplasmic intermediate filaments and, in muscle cells, accumulates at costameres (sarcolemmal adhesion structures) alongside dystrophin and spectrin.
• Post-translational modifications include phosphorylation and methylation, as identified by proteomics, suggesting regulatory roles in filament dynamics.
• KRT19 is also implicated in host-virus interactions, as highlighted by related studies, although the specific mechanisms remain to be fully elucidated.

Experimental Guidance and Technical Tips

• The immunogen comprises the C-terminal region of KRT19 (amino acids 243–400), which is expected to generate antibodies specific to the full-length protein; confirm this by running positive control lysates at the predicted molecular weight of ~44 kDa.
• For Western blotting, consider using total protein extracts from epithelial cell lines (e.g., HeLa, MCF-7) or tissues such as skin and liver, which express KRT19 at varying levels.
• In immunohistochemistry, antigen retrieval may be necessary for formalin-fixed paraffin-embedded sections; validate the protocol using positive control tissues like colon or breast carcinoma.
• The antibody's cross-reactivity with mouse and rat makes it potentially suitable for comparative studies; however, verify cross-reactivity in your specific model system as sequence homology might affect recognition.
• When performing immunofluorescence, KRT19 should display a cytoplasmic filamentous pattern; co-staining with nuclear and membrane markers can aid in subcellular localization analysis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibody pairs and bulk ancillary reagents. For cytoskeletal and epithelial research on targets like KRT19, we offer optimized monoclonal antibodies and sourcing services to accelerate your assay development. Our team can also assist in securing rare-target raw materials, ensuring a streamlined supply chain for your R&D and manufacturing needs.