Anti-Cytokeratin 18 (KRT18) Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P05783

Core Product Specifications and Parameters

Biological Background: Cytokeratin 18 (KRT18) Function and Localization

• KRT18 is a type I intermediate filament protein (also called cytokeratin‑18, CK‑18) that forms obligate heteropolymers with type II keratin KRT8, providing mechanical stability to simple epithelial cells. Related references: PMID:15529338 PMID:26823019
• KRT8/KRT18 filaments mediate ARHGEF40‑dependent actin stress fiber formation and reinforcement, and are required for the elongated morphology of epithelial tubules. Related references: PMID:26823019
• Functions downstream of ROCK kinase in a positive feedback mechanism in response to cellular mechanical stress. Related references: PMID:26823019
• Phosphorylation regulates filament reorganization. Related references: PMID:7523419 PMID:8522591 PMID:9298992
• Participates in the delivery of mutated CFTR to the plasma membrane and in IL‑6‑mediated barrier protection. Related references: PMID:15529338 PMID:16424149 PMID:17213200
• Localizes to the cytoplasm, perinuclear region, nucleus, nucleolus, and nuclear matrix. Expressed in colon, placenta, liver, and very weakly in exocervix; expression is increased in lymph nodes of breast carcinoma.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 127‑430 of human KRT18, covering the central rod domain; it may recognize both full‑length and proteolytic fragments in some samples.
• For Western blotting, a band of approximately 48‑50 kDa is expected; reduced migration can occur in highly phosphorylated states; consider phosphatase inhibitors during lysis.
• Cross‑reactivity with mouse and rat has been reported; however, sequence homology should be verified if using samples from other species.
• For IHC‑P and IF/ICC, fixation and antigen retrieval conditions should be optimized; paraffin sections may require mild retrieval.
• Always include positive and negative control lysates or tissues to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, technical services, and consulting for every stage from concept to clinic. This anti‑Cytokeratin 18 (KRT18) polyclonal antibody exemplifies our portfolio of high‑specificity reagents for intermediate filament and epithelial cell biology research. In addition to validated polyclonal antibodies, we offer matched antibody pairs, recombinant monoclonal antibodies, bulk ancillary reagents, and custom raw‑material sourcing for rare targets to streamline your assay development and production.