Anti-Cytokeratin 18 (CK18) Rabbit Monoclonal Antibody (WB, IF/ICC, IHC-P, ELISA) - P05783

Core Product Specifications and Parameters

Biological Background: Cytokeratin 18 Function and Localization

• Cytokeratin 18 (CK18), also known as Keratin-18 (K18) and cell proliferation-inducing gene 46 protein, is a type I intermediate filament protein. It forms obligate heteropolymers with type II keratin KRT8, providing mechanical stability and resilience to simple epithelial cells. Related references: PMID:15529338 PMID:26823019
• KRT8/KRT18 filaments are involved in ARHGEF40-mediated actin stress fiber formation and tensional force-induced stress fiber reinforcement. Related references: PMID:26823019
• Organization and orientation of KRT8/KRT18 filaments are responsible for the properly elongated morphology of epithelial tubules (By similarity).
• CK18 acts downstream of ROCK kinase activation as part of a positive feedback mechanism in response to cellular mechanical stress loading. Related references: PMID:26823019
• Phosphorylation of CK18 plays a role in filament reorganization. Related references: PMID:7523419 PMID:8522591 PMID:9298992
• CK18 is involved in the delivery of mutated CFTR to the plasma membrane. Related references: PMID:15529338 PMID:16424149
• CK18 is involved in interleukin-6 (IL-6)-mediated barrier protection. Related references: PMID:17213200
• Subcellularly, CK18 localizes to the nucleus matrix, cytoplasm, perinuclear region, and nucleolus. It is expressed in colon, placenta, liver, and weakly in exocervix; increased expression is observed in lymph nodes of breast carcinoma.

Experimental Guidance and Technical Tips

• The immunogen (aa 241-430) targets the conserved central coiled-coil domain of CK18, which likely contributes to the cross-reactivity observed with mouse and rat.
• For Western blot (WB), a single band at approximately 48 kDa is expected; validate using a relevant epithelial cell lysate.
• In immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), CK18 localizes to cytoplasmic filaments and perinuclear regions; appropriate fixation and permeabilization are critical.
• For ELISA applications, the antibody can be used as a capture or detection reagent; pairing with a suitable anti-CK18 antibody may enhance sensitivity.
• Given the monoclonal source (rabbit), lot-to-lot consistency is generally high, aiding reproducibility in diagnostic assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers by providing a comprehensive range of IVD raw materials, including this anti-CK18 monoclonal antibody optimized for multiple immunoassays. We also offer validated antibody pairs, recombinant antigens, and bulk ancillary reagents to streamline your assay development. For rare or custom targets, our sourcing team can help secure specialized raw materials, ensuring a reliable supply chain from concept to clinic.