Anti-Cytokeratin 17 Monoclonal Antibody for WB, IF, IHC, ELISA - Q04695

Core Product Specifications and Parameters

Biological Background: Cytokeratin 17 Function and Localization

• Cytokeratin 17, also known as keratin 17 (K17), is a type I intermediate filament protein and a member of the keratin family, characterized by a coiled-coil central rod domain.
• It plays a critical role in the formation and maintenance of skin appendages, particularly in determining hair shape and orientation, and is required for the persistence of the anagen (growth) phase of the hair follicle.
• K17 modulates the function of tumor necrosis factor-alpha (TNF-α) specifically in the context of hair cycling, influencing inflammatory and proliferative responses.
• The protein regulates epithelial cell growth and protein synthesis by binding to the adapter protein stratifin (SFN) and activating the Akt/mTOR signaling pathway.
• As a promoter of epithelial proliferation, K17 shapes the immune microenvironment by favoring a Th1/Th17-dominated response, thereby contributing to the development of basaloid skin tumors.
• In psoriasis, certain peptide regions of K17 have been identified as major targets for autoreactive T-cells, suggesting a role as an autoantigen in disease immunopathogenesis.
• Subcellularly, K17 is located in the cytoplasm, where it polymerizes with type II keratins to form intermediate filaments that provide mechanical support to cells.
• Tissue expression is prominent in the outer root sheath and medulla of hair follicles, digital pulp, nail matrix and bed, mucosal stratified squamous epithelia, basal cells of oral epithelium, palmoplantar epidermis, sweat and mammary glands, and myoepithelium of the prostate and urinary bladder.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a synthetic peptide spanning amino acids 333–432 of human K17. This region lies near the C-terminal tail domain, which may be less conserved across keratins; therefore, cross-reactivity with other keratin family members should be assessed.
• For Western blot, a band at approximately 48 kDa is expected; reducing conditions and adequate denaturation are recommended as K17 is a filament-forming protein.
• In immunofluorescence and IHC, permeabilization and antigen retrieval steps may be necessary to expose the cytoplasmic intermediate filament epitope. Validate using positive control tissues such as skin, hair follicle, or epithelial cell lines known to express K17.
• The antibody cross-reacts with mouse and rat, making it suitable for studies in common model organisms. However, verify reactivity in your specific assay conditions due to potential species-specific epitope variations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with a one-stop platform for IVD raw materials and technical services. As a trusted partner, we supply validated monoclonal antibodies like this anti-Cytokeratin 17 clone, along with optimized antibody pairs, bulk ancillary reagents, and custom rare-target sourcing. Whether your work focuses on epithelial biology, dermatopathology, or autoimmune research, CamelBio streamlines your procurement from concept to clinic.