Anti-Cytokeratin 17 (KRT17) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q04695

Core Product Specifications and Parameters

Biological Background: Cytokeratin 17 Function and Localization

• Cytokeratin 17 (KRT17), also known as 39.1, CK-17, or K17, is a type I keratin intermediate filament protein encoded by the KRT17 gene.
• Plays a critical role in the formation and maintenance of skin appendages, particularly in determining hair shape and orientation, and is required for the persistence of the anagen (growth) phase of hair follicles.
• Modulates the function of tumor necrosis factor (TNF) specifically in the context of hair cycling.
• Regulates protein synthesis and epithelial cell growth by binding to the adapter protein SFN and stimulating the Akt/mTOR pathway.
• Functions in tissue repair and may serve as a marker of basal cell differentiation in complex epithelia, indicative of a certain type of epithelial stem cell.
• Acts as a promoter of epithelial proliferation and an immune response regulator in skin, promoting a Th1/Th17-dominated immune environment that contributes to basaloid skin tumor development.
• May act as an autoantigen in psoriasis, with specific peptide regions serving as major targets for autoreactive T-cells, thereby contributing to disease immunopathogenesis.
• Localizes to the cytoplasm and is expressed in the outer root sheath of hair follicles, nail matrix, mucosal stratified squamous epithelia, basal cells of oral epithelium, palmoplantar epidermis, sweat glands, and mammary glands.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 133-432 of human KRT17, covering a large portion of the protein; expect the antibody to recognize total KRT17.
• Rabbit polyclonal antibodies are suitable for Western blot (WB), immunofluorescence/immunocytochemistry (IF/ICC), and enzyme-linked immunosorbent assay (ELISA).
• For WB, the predicted molecular weight is approximately 48 kDa; optimize loading and transfer conditions for clear detection.
• For IF/ICC, consider fixation and permeabilization protocols appropriate for cytoplasmic intermediate filament targets.
• Validate antibody performance in relevant sample types such as skin, hair follicle, nail matrix, or mucosal epithelial tissues.
• When using ELISA, include appropriate negative and positive controls to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting, covering every stage from concept to clinic. This Cytokeratin 17 polyclonal antibody supports research in dermatology, stem cell biology, and autoimmune diseases such as psoriasis. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to meet your procurement needs.