Anti-Cytokeratin 17 (KRT17) Monoclonal Antibody for WB, ELISA - Q04695

Core Product Specifications and Parameters

Biological Background: Cytokeratin 17 (KRT17) Function and Localization

• Keratin, type I cytoskeletal 17 (KRT17) is a type I keratin intermediate filament protein that plays a crucial role in the formation and maintenance of skin appendages, including hair follicles and nails, by determining their shape and orientation.
• KRT17 is essential for the correct growth of hair follicles, particularly by sustaining the anagen (growth) phase, and it modulates TNF-alpha signaling in the context of hair cycling.
• This protein is involved in tissue repair and may serve as a marker of basal cell differentiation in complex epithelia, indicating a certain type of epithelial stem cells.
• KRT17 promotes epithelial proliferation by acting as a regulator of immune responses in the skin, fostering a Th1/Th17-dominated immune environment that contributes to basaloid skin tumor development.
• It may act as an autoantigen in psoriasis, with specific peptide regions recognized by autoreactive T-cells, implicating it in the immunopathogenesis of the disease.
• Subcellularly, KRT17 is localized to the cytoplasm, consistent with its role as an intermediate filament component.
• Post-translational modifications include phosphorylation and ubiquitin conjugation, which may regulate its function and stability.
• The protein is expressed in various epithelial tissues, including the outer root sheath of hair follicles, nail bed, oral epithelium, palmoplantar epidermis, and glandular myoepithelium, as well as in the prostate, urinary bladder, sebaceous gland, and exocervix.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a synthetic peptide from the N-terminal region (aa 1-100) of human KRT17. For Western blotting, consider that the predicted molecular weight is approximately 48 kDa; validate in relevant human, mouse, or rat epithelial samples.
• For ELISA applications, the peptide immunogen itself may be useful as a positive control to assess antibody binding specificity.
• Cross-reactivity is reported for human, mouse, and rat; therefore, this antibody may be suitable for multi-species studies, but researchers should verify performance in their specific sample systems.
• As KRT17 is expressed in a wide range of epithelial tissues, whole-cell lysates from cultured keratinocytes or tissue extracts from skin, hair follicles, or glandular epithelia can be employed as positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic developers and researchers with high-quality antibodies, antigen proteins, and ancillary reagents. This anti-Cytokeratin 17 monoclonal antibody supports investigations into epithelial differentiation, hair follicle physiology, and autoimmune skin diseases such as psoriasis. Our portfolio also includes validated antibody pairs, bulk monoclonal and polyclonal antibodies, and custom rare-target material sourcing to streamline your assay development from concept to clinic.