Anti-Cytokeratin 16 (KRT16) Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P - P08779

Core Product Specifications and Parameters

Biological Background: Cytokeratin 16 Function and Localization

• KRT16 encodes a type I keratin protein, also known as Cytokeratin-16 (CK-16) or Keratin-16 (K16), that assembles into intermediate filaments in epithelial cells.
• It is an epidermis-specific keratin, playing a key role in maintaining skin structure and integrity.
• Acts as a regulator of innate immunity, providing an inflammatory checkpoint in response to skin barrier disruption.
• Expressed in the corneal epithelium at the protein level, highlighting its role in specialized epithelial tissues.
• KRT16 mutations are linked to ectodermal dysplasia and palmoplantar keratoderma, underscoring its clinical significance in dermatological disorders.
• Contains coiled-coil domains typical of intermediate filament proteins, facilitating dimerization and filament formation.
• Predicted molecular weight of approximately 51 kDa, consistent with its role as a structural protein.

Experimental Guidance and Technical Tips

• The immunogen (aa 190–430) encompasses the central rod domain of KRT16; therefore, the antibody is expected to recognize both full-length and potential processed forms. Validate specificity in the relevant sample system, such as human skin or corneal epithelium lysates.
• For Western blot, a primary signal near 51 kDa should be observed; consider using positive controls like HaCaT or primary keratinocyte extracts.
• For IHC-P and IF/ICC, standard antigen retrieval methods (e.g., citrate buffer, pH 6.0) are recommended; optimize antibody dilution and incubation conditions for your tissue type.
• Due to reported cross-reactivity with mouse and rat, this antibody may be suitable for rodent skin models; however, verify reactivity experimentally before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents. This anti-KRT16 antibody fits into dermatological and keratin biology research applications. Leverage CamelBio’s sourcing network to access rare target antibodies and streamline your assay development.