Anti-Cytokeratin 16 (KRT16) Monoclonal Antibody for WB - P08779

Core Product Specifications and Parameters

Biological Background: Cytokeratin 16 Function and Localization

• Cytokeratin 16 (KRT16) is a type I keratin, a member of the intermediate filament protein family, encoded by the KRT16 gene. It is also known as CK-16 or K16.
• KRT16 is an epidermis-specific protein that plays a structural role in skin and also acts as a key regulator of innate immunity in response to skin barrier breach, being required for certain inflammatory checkpoints in skin barrier maintenance.
• As a keratin, it localizes to the cytoplasmic intermediate filament network and forms obligate heterodimers with type II keratins to assemble the cytoskeleton.
• Expressed predominantly in the skin epithelium, with confirmed protein expression in the corneal epithelium, reflecting its role in protecting barrier tissues.
• Mutations in KRT16 are associated with ectodermal dysplasia, palmoplantar keratoderma, and other skin fragility disorders, highlighting its importance in mechanical integrity and cell resilience.
• The protein has a calculated molecular weight of approximately 51 kDa (51268 Da), and contains extensive coiled-coil domains necessary for filament formation.

Experimental Guidance and Technical Tips

• For Western blotting, expect a specific band around 51 kDa. Use lysates from skin or corneal epithelial cells as positive controls, and validate expression in your sample system.
• In immunohistochemistry (IHC-P), consider using heat-induced or protease-based antigen retrieval to expose the N-terminal epitope, and optimize antibody dilution depending on tissue and fixation method.
• For immunofluorescence (IF-P), anticipate a filamentous cytoskeletal staining pattern characteristic of intermediate filaments; methanol or formaldehyde fixation may be suitable.
• This monoclonal antibody was raised against a synthetic peptide corresponding to amino acids 1-100 of human KRT16; it is predicted to recognize full-length KRT16 and may cross-react with mouse KRT16 based on supplier data. Verify cross-reactivity in mouse models by Western blot or IHC before quantitative analyses.
• Due to its monoclonal nature, this reagent offers consistent lot-to-lot performance, which is critical for long-term diagnostic assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop procurement channel for IVD raw materials, bridging the gap from discovery to clinical application. With a focus on antibodies for skin biology and innate immunity targets like Cytokeratin 16, we offer validated monoclonal antibodies, optimized antibody pairs for immunoassays, and bulk ancillary reagents to support your diagnostic pipeline. From rare target raw-material sourcing to technical consultation, CamelBio streamlines your supply chain so you can accelerate product development with confidence.