Anti-Cytokeratin 14 (KRT14) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P02533

Core Product Specifications and Parameters

Biological Background: Cytokeratin 14 Function and Localization

• KRT14 is a type I intermediate filament protein essential for the structural framework of basal keratinocytes. It forms obligate heteropolymers with KRT5, establishing a resilient cytoskeletal network that provides mechanical strength to the epidermis and other stratified epithelia. Related references: PMID:11724817, PMID:1694855, PMID:1720261

• The nonhelical tail domain of KRT14 promotes the self-assembly of KRT5-KRT14 filaments into large bundles, enhancing filament resilience in vitro. Related references: PMID:11724817

• KRT14 is primarily cytoplasmic but can also localize to the nucleus. It is strongly expressed in the basal layer of stratified epithelia, including the epidermis, corneal epithelium, and outer root sheath of hair follicles, with decreased expression in suprabasal layers.

• Post-translational modifications such as phosphorylation, disulfide bond formation, and ubiquitination may regulate KRT14 filament dynamics and stability.

• Pathogenic variants in KRT14 are linked to skin fragility syndromes like epidermolysis bullosa simplex (EBS) and ectodermal dysplasia, underscoring its essential role in epithelial integrity.

• KRT14 expression is highly tissue-specific: it is present in basal keratinocytes and surrounding club hair during telogen, but absent from stratum corneum and inner root sheath.

Experimental Guidance and Technical Tips

• The immunogen spans the central rod domain of human KRT14 (aa 264-457); due to high homology, the antibody is expected to cross-react with mouse and rat KRT14, as indicated by the supplier. Always validate in the relevant species/tissue.

• For Western blot, a band at ~52kDa is expected; optimize loading and antibody dilution for clear detection in lysates from keratinocyte-rich tissues (e.g., skin, oral mucosa).

• For IHC-P, use formalin-fixed paraffin-embedded sections; antigen retrieval may be necessary. KRT14 staining marks basal cells; expect positive signal in the basal layer of epidermis and other stratified epithelia.

• In ELISA applications, use purified recombinant KRT14 protein as a standard and validate with known positive/negative samples.

• Since KRT14 forms complexes with KRT5, consider co-immunoprecipitation experiments to study keratin filament assembly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material sourcing, offering a comprehensive portfolio of validated monoclonal antibodies, antigen-grade proteins, and bulk ancillary reagents. This Cytokeratin 14 (KRT14) Rabbit mAb exemplifies our commitment to supporting keratinocyte and epithelial biology research, from biomarker discovery to diagnostic assay development. Whether you need optimized antibody pairs or hard-to-source targets, CamelBio bridges your supply chain seamlessly.