Anti-Cytokeratin 14 (KRT14) Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P02533

Core Product Specifications and Parameters

Biological Background: Cytokeratin 14 Function and Localization

• Keratin, type I cytoskeletal 14 (KRT14), also designated Cytokeratin-14 (CK-14) and Keratin-14 (K14), is a member of the type I keratin family of intermediate filament proteins.
• KRT14 forms obligate heteropolymers with the type II keratin KRT5, contributing to the keratin intermediate filament network that confers mechanical strength and structural integrity to the basal layer of the epidermis and other stratified epithelia. Related references: PMID:11724817 PMID:1694855 PMID:1720261
• The nonhelical tail domain of KRT14 promotes KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical resilience of keratin intermediate filaments in vitro. Related references: PMID:11724817
• Subcellularly, KRT14 localizes to both the cytoplasm and nucleus, consistent with its role in maintaining cytoskeletal architecture and potential nuclear functions.
• Tissue expression is prominent in the basal keratinocytes of stratified epithelia: detected in the corneal epithelium, basal epidermal layer, and outer root sheath of anagen hair follicles, but absent from terminally differentiated suprabasal layers such as the stratum corneum.
• Genetic variations in KRT14 are associated with multiple heritable skin fragility disorders, including epidermolysis bullosa simplex, ectodermal dysplasia, and palmoplantar keratoderma.
• The protein undergoes post-translational modifications including phosphorylation and ubiquitination, and contains coiled-coil domains and disulfide bonds essential for filament assembly and stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 371-470 within the C-terminal nonhelical tail domain; the antibody may preferentially recognize epitopes in this region critical for filament bundling and mechanical resilience.
• For Western blot, a band near 52 kDa is expected in keratinocyte lysates; validate band identification using positive controls (e.g., HaCaT cell lysate) and consider that post-translational modifications may cause slight migration shifts.
• In immunohistochemistry (paraffin) and immunofluorescence, strong cytoplasmic and nuclear staining is anticipated in basal epithelial cells; skin, cornea, or oral mucosa sections are suitable positive control tissues.
• Reported cross-reactivity with human, mouse, and rat suggests conserved epitopes; however, for use in other species or applications requiring high specificity, sequence alignment of the immunogen region is recommended.
• As a polyclonal antibody, lot-to-lot consistency may vary; validate each new lot in your assay system, especially for quantitative applications like ELISA.
• For ELISA, the synthetic peptide immunogen can serve as a coating antigen for antibody titration or as a positive control to assess plate-bound activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio positions itself as a comprehensive partner for IVD raw-material procurement, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. This anti-Cytokeratin 14 polyclonal antibody is a valuable tool for investigating epidermal biology, skin diseases, and keratin-related pathologies. We offer one-stop access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing—streamlining your supply chain for keratin and epithelial research.