Anti-Cytokeratin 13 (KRT13) Rabbit Polyclonal Antibody for WB, ELISA - P13646

Core Product Specifications and Parameters

Biological Background: Cytokeratin 13 Function and Localization

• Keratin, type I cytoskeletal 13 (KRT13) is an intermediate filament protein specifically expressed in mucosal stratified squamous epithelia.
• It forms obligate heteropolymers with type II keratin KRT4 to assemble keratin intermediate filament networks, providing mechanical integrity to mucosal tissues (PubMed:7493031). Related references: PMID:7493031
• KRT13 contributes to postnatal tongue mucosal cell homeostasis and tissue organization in response to mechanical stress, potentially via regulating the G1/S phase cyclins CCNE1 and CCNE2 (By similarity).
• At the protein level, KRT13 is detected in epidermal sweat gland ducts, exocervix, esophagus, and placenta.
• The protein undergoes various post-translational modifications, including phosphorylation, methylation, and glycosylation, which may regulate filament dynamics.
• Alternative splicing produces multiple isoforms, and mutations in KRT13 are associated with disease variants.
• KRT13 belongs to the type I keratin family, characterized by a central coiled-coil rod domain critical for filament assembly.
• Its calculated molecular weight is approximately 49.6 kDa, consistent with the observed band around 50 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 269–458 of human KRT13 (NP_705694.2), covering the C-terminal region of the protein. This region includes part of the rod domain and the tail, which may offer good accessibility for antibody binding.
• For Western blot, the antibody detects a band at ~50 kDa in human, mouse, and rat samples. Consider using appropriate positive controls (e.g., esophagus or exocervical lysates) and validate in your specific model system. Titrate the primary antibody to determine the optimal dilution for your assay.
• For ELISA, optimization of coating antigen concentration, blocking conditions, and detection reagents is recommended. The polyclonal nature may contribute to robust signal but batch-to-batch consistency should be assessed.
• Given cross-reactivity with mouse and rat, this antibody can be used for non-human primate or rodent studies. Confirm reactivity with your sample species if not listed.
• Since keratins are prone to aggregation, use mild reducing conditions and avoid excessive boiling of samples to preserve epitopes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by connecting diagnostic manufacturers and laboratories with validated antibody products like this anti-Cytokeratin 13 (KRT13) polyclonal antibody. Beyond individual antibodies, we support mucosal epithelial and keratin research with comprehensive sourcing options—including validated antibody pairs, bulk ancillary reagents, and customized rare-target raw materials—to help accelerate your diagnostic development and translational projects.