Anti-Cytokeratin 13 (KRT13) Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P13646

Core Product Specifications and Parameters

Biological Background: Cytokeratin 13 (KRT13) Function and Localization

• Cytokeratin 13 (KRT13) is a type I keratin encoded by the KRT13 gene and is also known as CK13 or K13.
• It functions as a structural component of intermediate filaments in mucosal stratified squamous epithelia. Related references: PMID:7493031
• KRT13 forms heteropolymeric keratin intermediate filament networks with the type II keratin KRT4, providing mechanical stability to the mucosal epithelium. Related references: PMID:7493031
• The protein contributes to postnatal tongue mucosal cell homeostasis and tissue organization in response to mechanical stress, potentially through regulation of the G1/S phase cyclins CCNE1 and CCNE2 (By similarity).
• According to UniProt, KRT13 is expressed in epidermal sweat gland ducts, exocervix, esophagus, and placenta.
• Post-translational modifications include phosphorylation, methylation, and glycosylation, as indicated by UniProt keywords.
• Alternative splicing generates multiple transcript variants.
• The calculated molecular weight from the UniProt canonical sequence is approximately 49.6 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 100-200, which lies within the N-terminal rod domain. This region may be conserved across species, consistent with observed cross-reactivity with mouse and rat.
• For Western blotting, consider using whole-cell lysates from mucosal epithelial cell lines or tissues and validate detection of the expected ~50 kDa band with appropriate controls.
• In immunofluorescence and immunohistochemistry (paraffin), proper antigen retrieval and permeabilization may be necessary to expose the intermediate filament network. Validate staining patterns in known positive tissues (e.g., esophagus, cervix).
• The antibody may also detect splice variants or cleavage fragments; confirm specificity using siRNA knockdown or knockout controls where possible.
• For ELISA applications, the peptide immunogen can serve as a positive control for coating or competition assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-Cytokeratin 13 (KRT13) monoclonal antibody is part of our extensive portfolio for epithelial biology and mucosal disease research. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development and manufacturing workflows.