Anti-Cytokeratin 12 (KRT12) Rabbit Polyclonal Antibody for WB and ELISA - Q99456

Core Product Specifications and Parameters

Biological Background: Cytokeratin 12 (KRT12) Function and Localization

• Encodes a type I keratin intermediate filament protein, keratin 12 (K12), which pairs with the type II keratin KRT3 to form heteropolymeric filaments in the corneal epithelium.
• Essential for providing mechanical strength and structural integrity to the corneal epithelial layer, thereby protecting the eye surface.
• Expression is restricted to the corneal epithelium, as confirmed at the protein level, making it a reliable marker for corneal epithelial cells.
• Mutations in KRT12 are associated with Meesmann corneal dystrophy (MECD1), an autosomal dominant disorder characterized by corneal epithelial microcysts.
• Belongs to the keratin family of intermediate filament genes, critical for the cytoarchitecture and resilience of epithelial tissues.
• The protein undergoes post-translational modifications and has been identified through direct protein sequencing and mass spectrometry-based proteomics.
• Predicted molecular weight from UniProt is approximately 53.5 kDa (53511 Da), consistent with the observed 54 kDa product weight.
• As an intermediate filament protein, KRT12 predominantly localizes to the cytoplasm of corneal epithelial cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 295-494) of human KRT12, encompassing part of the rod and tail domains. This region may confer specificity over other keratins; specificity should be confirmed using recombinant proteins or appropriate controls.
• For Western blot, a band at approximately 54 kDa is expected. Use positive control lysates from human corneal epithelial cells or tissue extracts. Cross-reactivity with mouse and rat suggests potential utility in rodent corneal studies, but validation in those species is recommended.
• In ELISA, the antibody can be employed for direct or indirect detection. Optimize coating conditions and antibody dilution experimentally. When developing a sandwich ELISA, pairing with a KRT12-specific capture antibody may improve performance.
• As a polyclonal antibody, batch-to-batch variability is possible. Consistent performance should be verified using standardized protocols and internal controls.
• Applications beyond WB and ELISA (e.g., immunofluorescence, immunohistochemistry) are not listed in the specifications and would require thorough validation by the end user.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner for diagnostic manufacturers, labs, and research institutes, providing validated antibodies for corneal disease markers and beyond. Our one-stop platform offers antibody pairs, optimized polyclonal reagents, and ancillary materials to accelerate assay development from concept to clinic. For KRT12-related research and corneal diagnostics, we can facilitate customized raw-material procurement and technical consultation.