Anti-Cytokeratin 12 (KRT12) Rabbit Polyclonal Antibody for WB and ELISA - Q99456

Core Product Specifications and Parameters

Biological Background: Keratin 12 Function and Localization

• Keratin 12 is a type I intermediate filament protein that heterodimerizes with type II keratin KRT3 to form keratin intermediate filaments specifically in the corneal epithelium.
• It provides mechanical strength and structural integrity to the corneal epithelium, essential for maintaining corneal transparency and protection.
• As a member of the keratin family, KRT12 contains a central coiled-coil rod domain, characteristic of intermediate filament proteins.
• Mutations in KRT12 are associated with Meesmann corneal dystrophy, an autosomal dominant disorder characterized by microcysts in the corneal epithelium.
• The protein is specifically expressed in the corneal epithelium, as confirmed by proteomics identification and direct protein sequencing.
• Additional keywords include coiled coil, intermediate filament, disease variant, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen used spans the C-terminal region (amino acids 295–494) of human KRT12; this antibody may therefore recognize full-length KRT12 and its relevant fragments.
• For Western blot, consider using corneal epithelial cell lysates as a positive control; the predicted molecular weight is approximately 54 kDa.
• In ELISA applications, validate the antibody against the recombinant immunogen or native KRT12 under your specific experimental conditions.
• Due to reported cross-reactivity with mouse and rat, this antibody may be suitable for studies in rodent corneal tissues; however, perform validation in your target sample.
• For immunohistochemistry or other applications, determine optimal dilution and conditions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes by providing a comprehensive range of IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies for keratins and corneal biomarkers. Our portfolio covers bulk ancillary reagents and rare target sourcing, helping you move efficiently from concept to clinic. For cytokeratin 12 research, we offer tailored procurement solutions to secure the critical reagents you need.