Anti-Cytokeratin 12 (KRT12) Rabbit Monoclonal Antibody for IF-P, IHC-P, ELISA - Q99456

Core Product Specifications and Parameters

Biological Background: Cytokeratin 12 (KRT12) Function and Localization

• Keratin 12 (KRT12) is a type I intermediate filament protein, also known as cytokeratin-12 or CK-12, encoded by the KRT12 gene (Gene ID: 3859).
• It functions as a structural component of intermediate filaments in the corneal epithelium, forming obligate heterodimers with the type II keratin KRT3 to assemble the keratin intermediate filament network.
• KRT12 is specifically expressed in the corneal epithelium, where it provides mechanical strength and maintains cellular integrity.
• Mutations in KRT12 are linked to Meesmann corneal dystrophy (MECD1), an autosomal dominant disorder characterized by microcysts in the corneal epithelium.
• Subcellularly, KRT12 localizes to the cytoplasmic keratin filament network.
• The protein has a predicted molecular weight of 54 kDa (UniProt: 53511 Da).

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 250–494 of human KRT12, spanning part of the central rod domain, which may enable recognition of both native and denatured protein forms.
• For immunofluorescence and immunohistochemistry, consider using corneal epithelium sections or cell lines, where cytoplasmic filamentous staining is anticipated.
• Cross-reactivity with mouse and rat has been observed; verify performance in your specific experimental system.
• Optimize antibody dilution and assay conditions (e.g., antigen retrieval for IHC) according to standard laboratory protocols.
• This antibody is suitable for IF-P, IHC-P, and ELISA applications; validate sensitivity and specificity in your target tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, providing diagnostic developers and research teams with access to validated antibodies such as this anti-KRT12 clone for corneal disease and keratin research. Our portfolio includes optimized monoclonal and polyclonal antibodies, matched antibody pairs, and bulk ancillary reagents, alongside custom procurement services for rare targets. We support the entire workflow from early-stage R&D to clinical application, streamlining the path from concept to clinic.