Anti-Cytokeratin 10 Monoclonal Antibody for IF-P, ELISA - P13645

Core Product Specifications and Parameters

Biological Background: Cytokeratin 10 Function and Localization

• Keratin 10 (CK-10, K10) is a type I intermediate filament protein encoded by the KRT10 gene.
• In suprabasal keratinocytes, CK10 forms heteropolymers primarily with the type II keratin KRT1, contributing to the intermediate filament network essential for epidermal differentiation, mechanical integrity, and barrier function.
• Belongs to the keratin family, characterized by a central α-helical rod domain flanked by non-helical head and tail domains; post-translationally modified by phosphorylation and disulfide bond formation.
• While primarily cytoplasmic as part of the cytoskeleton, CK10 is also detected in the extracellular space and on the cell surface, particularly in differentiated epithelial cells.
• Expressed in all suprabasal layers of stratified epithelia, including the stratum corneum. Additionally, CK10 is found on the surface of desquamated nasal epithelial cells and lung cell lines. Related references: PMID:12427098 PMID:19627498
• CK10 serves as a receptor for Staphylococcus aureus on desquamated nasal epithelial cells, interacting with clfB and thereby promoting nasal colonization. Related references: PMID:12427098
• CK10 binds Streptococcus pneumoniae PsrP on lung cell lines, facilitating bacterial adherence; this interaction does not require glycosylation of either protein. Related references: PMID:19627498

Experimental Guidance and Technical Tips

• Immunogen design: The antibody was raised against a synthetic peptide spanning the C-terminal region (amino acids 485–584) of human CK10, which is predicted to be specific to the CK10 sequence and less conserved among other keratins.
• Application suitability: This antibody is suitable for immunofluorescence on fixed/permeabilized samples (IF-P) and ELISA. For IF-P, optimize fixation and permeabilization to preserve intermediate filament structure; for ELISA, titrate antibody and validate in the relevant sample matrix.
• Cross-reactivity: The antibody shows reactivity with human, mouse, and rat CK10, making it useful for studies in rodent models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing solution for IVD raw materials, including this Cytokeratin 10 antibody and complementary epithelial marker reagents. From validated antibody pairs to bulk ancillary reagents, we support your assay development needs. Contact us to simplify your path from research to clinical application.