Anti-Cytokeratin 1 (KRT1) Rabbit Monoclonal Antibody for WB, IF-P, ELISA - P04264

Core Product Specifications and Parameters

Biological Background: Cytokeratin 1 (KRT1) Function and Localization

• Keratin, type II cytoskeletal 1 (KRT1), also designated cytokeratin-1 (CK-1) or 67 kDa cytokeratin, is a type II intermediate filament protein encoded by the KRT1 gene in humans.
• As a structural component of intermediate filaments, KRT1 forms heteropolymers with type I keratins, providing mechanical strength and stability to differentiating suprabasal keratinocytes of stratified epithelia. Related references: PMID:1381288
• Through interactions with integrin beta-1 (ITB1) and receptor of activated protein C kinase 1 (RACK1), KRT1 may regulate the activity of kinases such as PKC and SRC. Related references: PMID:17956333
• In complex with C1QBP, KRT1 serves as a high-affinity receptor for kininogen-1 (HMWK), linking intermediate filaments to cell signaling. Related references: PMID:21544310
• Subcellularly, KRT1 localizes to both the cytoplasm and the cell membrane, consistent with its structural and receptor functions.
• KRT1 is predominantly expressed in terminally differentiating epidermis; the protein source for characterization is often neonatal foreskin.
• This keratin undergoes several post-translational modifications, including phosphorylation, methylation, and citrullination, and is associated with skin disorders such as ichthyosis and palmoplantar keratoderma when mutated.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 545–644 in the C-terminal region of human KRT1. This region is likely surface‑exposed in assembled filaments, making the antibody suitable for detection of both denatured and native KRT1 under appropriate conditions.
• For Western blotting (WB), the predicted molecular weight of KRT1 is approximately 66 kDa. Include proper reducing agents and denaturing steps; validate detection in your specific cell or tissue lysates, as keratin extraction may require high‑salt or urea buffers.
• In immunofluorescence of paraffin‑embedded sections (IF‑P), antigen retrieval is essential. A permeabilization step may improve accessibility of the intracellular target. Confirm reactivity with human, mouse, or rat skin specimens.
• For ELISA, pair this monoclonal antibody with an appropriate type I keratin detection antibody if developing a sandwich immunoassay. Titrate both capture and detection reagents in your assay system.
• As cross‑reactivity is noted for human, mouse, and rat, consider sequence alignment of the immunogen region to verify epitope conservation and employ species‑specific controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined one-stop access to premium IVD raw materials, including this anti-KRT1 monoclonal antibody tailored for keratinocyte and dermatological research. From validated antibody pairs and bulk ancillary reagents to rare‑target custom sourcing, CamelBio bridges the gap from concept to clinic with comprehensive technical and supply support.