Anti-Cytochrome P450 4A (CYP4A11) Monoclonal Antibody for WB, IHC-P, ELISA - Q02928

Core Product Specifications and Parameters

Biological Background: Cytochrome P450 4A11 (CYP4A11) Function and Localization

• Cytochrome P450 4A11 (CYP4A11), also termed 20-hydroxyeicosatetraenoic acid (20-HETE) synthase, fatty acid omega-hydroxylase, and CYPIVA11, is a heme-thiolate monooxygenase belonging to the cytochrome P450 family.
• As a monooxygenase, CYP4A11 catalyzes the ω-oxidation of various fatty acids by incorporating one oxygen atom into the substrate while reducing the other to water, utilizing NADPH and cytochrome P450 reductase as electron donors. Related references: PMID:10553002 PMID:10660572 PMID:15611369
• In the liver, CYP4A11 acts as the principal ω-hydroxylase for lauric acid (dodecanoic acid), with decreasing catalytic efficiency toward tetradecanoic, oleic, linoleic, and hexadecanoic acids. Related references: PMID:15611369 PMID:1739747 PMID:7679927
• The enzyme metabolizes arachidonic acid to 20-HETE, a potent signaling lipid that regulates vasoconstriction, natriuresis, and arterial blood pressure, thus contributing to cardiovascular homeostasis. Related references: PMID:10620324 PMID:10660572 PMID:15611369
• Additionally, CYP4A11 can perform ω-1 hydroxylation of fatty acids at lower efficiency and may participate in the chain-shortening degradation of very long-chain fatty acids (e.g., docosanoic acid) via successive ω-oxidations. Related references: PMID:7679927 PMID:18182499
• Subcellularly, CYP4A11 localizes to the endoplasmic reticulum and microsomal membranes, with high tissue expression in human liver and the S2/S3 segments of renal proximal tubules. Related references: PMID:7679927 PMID:10660572
• The protein is subject to alternative splicing and contains key functional motifs including heme- and iron-binding sites essential for its monooxygenase activity.

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody was raised against a synthetic peptide within amino acids 50-150 of human CYP4A11; it may detect full-length and certain splice variants—validate in your specific experimental context.
• For Western blot, expect a band around 59 kDa; consider titrating the antibody and optimizing blocking and detection conditions for your lysate type.
• In IHC-P, expression in liver and kidney is expected; standard antigen retrieval (e.g., citrate buffer, pH 6.0) and appropriate tissue fixation can influence staining—perform pilot tests.
• For ELISA, the antibody can be used as a capture or detection reagent; pairing with a matched antibody pair or recombinant protein standard is advisable for quantitative assays.
• Cross-reactivity with mouse and rat CYP4A isoforms is predicted, but due to sequence differences, confirm specificity in your model system, especially in knockout or knockdown controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, from validated antibodies like this anti-CYP4A11 monoclonal antibody to complementary reagents for lipid metabolism and hypertension studies. Leverage our supply capabilities—including optimized antibody pairs, bulk ancillary reagents, and custom rare-target development—to streamline your fatty acid oxidation, 20-HETE, or drug metabolism assay pipelines from concept to clinical application.