Anti-Cytochrome P450 2E1 (CYP2E1) Rabbit mAb for WB, IHC-P, ELISA - P05181

Core Product Specifications and Parameters

Biological Background: Cytochrome P450 2E1 (CYP2E1) Function and Localization

• CYP2E1, also designated CYPIIE1, P450-J, or 4-nitrophenol 2-hydroxylase, is a member of the cytochrome P450 superfamily of heme-thiolate monooxygenases.
• It functions as a cytochrome P450 monooxygenase that metabolizes fatty acids through hydroxylation at the omega-1 position, utilizing molecular oxygen and electrons from NADPH via cytochrome P450 reductase. Related references: PMID:10553002 PMID:18577768
• CYP2E1 may also be involved in the oxidative metabolism of xenobiotics (Probable).
• The protein localizes to the endoplasmic reticulum (microsomal) membrane and the mitochondrial inner membrane.
• It contains a heme prosthetic group and binds iron, which are essential for its catalytic activity.
• Encoded by the CYP2E1 gene (GeneID: 1571), the protein has a molecular weight of approximately 57 kDa.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 234-493 of human CYP2E1 (NP_000764.1), a region that may confer high specificity; consider validating reactivity in your target species.
• In WB applications, expect a band near 57 kDa; optimize primary antibody dilution and incubation times for your specific sample.
• For IHC-P, antigen retrieval methods may be necessary to expose the membrane-bound target; recommend testing on positive control tissues such as liver.
• For ELISA, use a standard sandwich ELISA format with a matched antibody pair and validate with known CYP2E1 standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For targets like CYP2E1 involved in liver function and xenobiotic metabolism, we provide one-stop sourcing from early research to clinical assay development. Whether you need custom antibodies or rare-target raw materials, our integrated supply chain ensures reliable, high-quality delivery for your diagnostic pipeline.