Anti-Cytochrome P450 2E1 (CYP2E1) Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P05181

Core Product Specifications and Parameters

Biological Background: Cytochrome P450 2E1 Function and Localization

• Cytochrome P450 2E1 (CYP2E1), also known as CYPIIE1 or P450-J, is a member of the cytochrome P450 superfamily encoded by the CYP2E1 gene (Gene ID: 1571).
• It catalyzes the hydroxylation of carbon-hydrogen bonds, specifically mediating omega-1 hydroxylation of fatty acids, with highest catalytic activity for saturated fatty acids (PubMed:10553002, PubMed:18577768). Related references: PMID:10553002 PMID:18577768
• Mechanistically, CYP2E1 uses molecular oxygen and NADPH via cytochrome P450 reductase to insert one oxygen atom into substrates while reducing the second into water (PubMed:10553002, PubMed:18577768). Related references: PMID:10553002 PMID:18577768
• CYP2E1 may be involved in the oxidative metabolism of xenobiotics, including drugs and environmental toxins, contributing to their bioactivation or detoxification.
• Subcellular localization includes the endoplasmic reticulum membrane, microsome membrane, and mitochondrial inner membrane, reflecting its roles in fatty acid and xenobiotic metabolism.
• The protein contains a heme iron-binding motif and is classified as an oxidoreductase acting on paired donors with incorporation of molecular oxygen.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 234-493 of human CYP2E1, covering the C-terminal region; this may yield antibodies recognizing epitopes in that domain.
• For Western blot, the predicted molecular weight is ~57 kDa (UniProt: 56.8 kDa); ensure proper sample preparation to maintain membrane protein integrity.
• In immunofluorescence and immunohistochemistry, CYP2E1 staining is expected in cytoplasmic and membranous compartments; validate signal specificity with appropriate controls.
• The antibody cross-reacts with mouse and rat CYP2E1, enabling multi-species studies; consider species-specific optimization of assay conditions.
• For ELISA, consider using this monoclonal antibody as a capture or detection reagent in paired antibody strategies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering this anti-CYP2E1 monoclonal antibody for research in drug metabolism and toxicology applications. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your diagnostic development from concept to clinic.