Anti-CYTH4 Polyclonal Antibody for WB, ELISA - Q9UIA0

Core Product Specifications and Parameters

Biological Background: Cytohesin-4 Function and Localization

• Cytohesin-4 is a member of the cytohesin family that functions as a guanine-nucleotide exchange factor (GEF) for ADP-ribosylation factor (ARF) proteins.
• It specifically activates ARF1 and ARF5 by promoting the exchange of GDP for GTP.
• The protein contains a pleckstrin homology (PH) domain, a SEC7 domain, and a coiled-coil domain, as reflected by its alternate name PH, SEC7 and coiled-coil domain-containing protein 4.
• Cytohesin-4 localizes to the cell membrane, consistent with its role in ARF-mediated membrane trafficking.
• It is predominantly expressed in peripheral blood leukocytes, suggesting a specialized function in immune cells.
• The protein is annotated with keywords including cell membrane, coiled coil, guanine-nucleotide releasing factor, lipid-binding, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal 70 amino acids of human CYTH4, which includes portions of the PH and coiled-coil domains; consider that epitope accessibility may vary across applications.
• For Western blot, expect a band at approximately 46 kDa; validate using lysates from human peripheral blood leukocytes or CYTH4-transfected cells.
• In ELISA, titrate the antibody against the recombinant immunogen or full-length CYTH4 protein to determine optimal working concentration.
• Reported cross-reactivity with mouse and rat should be confirmed by testing on relevant species samples under experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw material sourcing, supporting projects from concept to clinic. For CYTH4-related ARF signaling and immunology research, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your assay development. Our one-stop model ensures efficient procurement of critical reagents for diagnostic manufacturers and research laboratories alike.