Anti-Cystatin C Polyclonal Antibody for WB, ELISA - P01034

Core Product Specifications and Parameters

Biological Background: Cystatin C Function and Localization

• Cystatin C (CST3 gene) encodes a secreted cysteine protease inhibitor, also known as Cystatin-3, Gamma-trace, and Post-gamma-globulin.
• It inhibits cysteine proteinases, regulating their activity locally in tissues and body fluids.
• The protein is secreted and widely distributed in body fluids, including cerebrospinal fluid, plasma, and saliva.
• Tissue expression is highest in epididymis, vas deferens, brain, thymus, and ovary, with lower levels in the submandibular gland.
• Cystatin C is implicated in pathological conditions such as age-related macular degeneration, amyloidosis, and leukodystrophy, as reflected by its UniProt keywords.
• Structurally, it contains disulfide bonds and undergoes glycosylation and phosphorylation, contributing to its stability and function.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fusion protein spanning amino acids 27-146 of human Cystatin C (NP_000090.1), covering the mature protein region. This immunogen design ensures recognition of the secreted form.
• For WB, the predicted molecular weight is approximately 13 kDa, but the observed band may appear slightly higher due to glycosylation; validate in relevant sample systems such as human plasma or tissue lysates.
• In ELISA, consider pairing with a matched detection antibody for sandwich assays; note that Cystatin C is secreted, so cell culture supernatants or biological fluids are suitable samples.
• Due to cross-reactivity with mouse and rat, this antibody can be used in translational studies across species; confirm specificity with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, including validated antibodies, reagents, and technical support from concept to clinic. For Cystatin C research, we offer polyclonal and monoclonal antibodies, antigen proteins, and ancillary reagents tailored for immunoassay development. Our team can assist with sourcing rare targets and providing bulk supply solutions.