Anti-Cystatin B Monoclonal Antibody for WB, ELISA - P04080

Core Product Specifications and Parameters

Biological Background: Cystatin B Function and Localization

• Cystatin B (also known as Stefin-B, CPI-B, or liver thiol proteinase inhibitor) is an intracellular protein belonging to the cystatin superfamily of cysteine protease inhibitors.
• It functions as a tight-binding reversible inhibitor of cathepsins L, H, and B, thereby regulating lysosomal cysteine protease activity.
• The protein is encoded by the CSTB gene (also designated CST6 or STFB) and is located on human chromosome 21.
• Subcellular localization includes both the cytoplasm and the nucleus, consistent with its role in controlling proteolysis in multiple compartments.
• Post-translational modifications include acetylation, and the protein has been identified through direct protein sequencing and proteomics approaches.
• Mutations in CSTB are associated with progressive myoclonus epilepsy (EPM1), a neurodegenerative disorder, highlighting its role in neuronal function.
• The mature protein has a molecular weight of approximately 11 kDa, as determined by sequence analysis.
• Additional keywords linking to disease variants include epilepsy and neurodegeneration, underscoring its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering the N-terminal region (amino acids 1–98) of full-length human Cystatin B, suggesting the antibody likely recognizes the native protein.
• For Western blot analysis, a band around 11 kDa is expected; consider using 12–15% SDS-PAGE gels and optimized transfer conditions for small proteins.
• Cross-reactivity with mouse and rat Cystatin B has been reported; validation in the relevant sample system is recommended.
• In ELISA, coating antigen and antibody concentration should be titrated to achieve optimal signal-to-noise ratio; this antibody is suitable for both direct and sandwich ELISA formats.
• Since Cystatin B is itself a protease inhibitor, standard sample preparation may not require additional thiol protease inhibitors, but confirm experimentally.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated one-stop sourcing platform, CamelBio provides diagnostic manufacturers and research laboratories with consistent access to critical IVD raw materials, including this high-quality anti-Cystatin B monoclonal antibody. Whether you need validated antibody pairs for protease-related assays, bulk ancillary reagents, or rare target sourcing, CamelBio supports your workflow from early-stage development to clinical application. For targets involved in neurodegeneration and protease inhibition pathways, our team can assist in selecting optimized immunoreagents to accelerate your diagnostic kit development.