Anti-Cystathionine Beta-Synthase (CBS) Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P35520

Core Product Specifications and Parameters

Biological Background: Cystathionine Beta-Synthase Function and Localization

• Cystathionine beta-synthase (CBS), also known as beta-thionase or serine sulfhydrase, is encoded by the CBS gene and exists in several isoforms due to alternative splicing.
• CBS catalyzes the first step of the transsulfuration pathway, converting L-serine and L-homocysteine into L-cystathionine, which is crucial for L-cysteine biosynthesis and detoxification of homocysteine. Related references: PMID:20506325 PMID:23974653 PMID:23981774
• The enzyme contributes to the production of hydrogen sulfide (H₂S), a gasotransmitter involved in neuronal signaling and cytoprotection.
• CBS is a pyridoxal-phosphate-dependent enzyme that also contains a heme-binding domain and regulatory CBS domains, enabling allosteric regulation.
• It is localized in both the cytoplasm and nucleus, supporting its metabolic and potential regulatory functions.
• Highest expression is observed in liver and pancreas, with variable expression in other tissues, and particular importance during fetal development in brain, liver, and kidney.
• Post-translational modifications, including phosphorylation and ubiquitination, and disease-associated mutations underline its clinical significance in homocystinuria.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 400–551 of human CBS, a C-terminal region that may be present in multiple splice variants; verify isoform specificity in your experimental system.
• For Western blotting, a band at ~61 kDa is expected in lysates from tissues with high CBS expression (e.g., liver, pancreas); consider using these as positive controls.
• In immunohistochemistry (IHC-P), optimize antigen retrieval and antibody dilution using known positive tissues such as liver or kidney sections to account for variable antigen accessibility.
• For immunofluorescence and ELISA assays, validate the antibody’s performance with recombinant CBS protein or overexpressing cell lines to ensure sensitivity and specificity.
• Cross-reactivity with mouse or rat CBS has not been reported; verify reactivity if using non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with comprehensive access to IVD raw materials, including this anti-CBS monoclonal antibody for applications in homocysteine metabolism and hydrogen sulfide signaling studies. Our supply portfolio spans validated antibody pairs, bulk reagents, and custom sourcing solutions to streamline your assay development from concept to clinic. Partner with us to secure rare target raw materials and optimize your workflow.