Anti-Cystathionase/CTH Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P32929

Core Product Specifications and Parameters

Biological Background: Cystathionase/CTH Function and Localization

• Cystathionine gamma-lyase (CTH/CSE/CGL) is a PLP-dependent enzyme that cleaves L-cystathionine into L-cysteine, ammonia, and 2-oxobutanoate — the final step of the trans-sulfuration pathway. Related references: PMID:10212249, PMID:18476726, PMID:19261609
• The L-cysteine produced by CTH is utilized for glutathione biosynthesis, a critical antioxidant. Related references: PMID:18476726
• CTH generates the gasotransmitter hydrogen sulfide (H2S) from L-cysteine or L-homocysteine, which plays key roles in vasodilation, bone protection, and inflammation. Related references: PMID:10212249, PMID:19019829, PMID:19261609, PMID:29254196
• Under hyperhomocysteinemia, CTH can produce homolanthionine and H2S from homocysteine, and lanthionine and H2S from cysteine — reactions with potential pathological relevance. Related references: PMID:19261609
• CTH mediates protein sulfhydration, converting -SH to -SSH on targets like GAPDH, PTPN1, and RELA, modulating their function. Related references: PMID:22169477
• CTH is primarily cytoplasmic and highly expressed in liver and muscle tissues, with low expression in most other tissues. Related references: PMID:10727430, PMID:20305127
• The enzyme is calmodulin-binding, PLP-dependent, and subject to alternative splicing; disease-associated variants have been reported.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein covering the full-length human CTH sequence (aa 1-405), suggesting the polyclonal antibody may recognize multiple linear and conformational epitopes.
• For Western blotting, a single band around 45 kDa is expected; consider using liver or muscle lysates as positive controls due to high CTH expression.
• Cross-reactivity with mouse and rat indicates conserved epitopes; validation in these species is recommended using appropriate tissue controls.
• In immunofluorescence/ICC, cytoplasmic staining is anticipated; optimize fixation/permeabilization conditions to preserve epitope accessibility.
• For immunoprecipitation, the antibody may effectively pull down CTH and associated protein complexes; validate with relevant sample systems.
• ELISA can be employed for quantitative detection of CTH; pairing with a detection antibody may enhance sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined sourcing solution for IVD raw materials, including validated polyclonal antibodies like this anti-CTH antibody. Whether you require optimized antibody pairs, bulk ancillary reagents, or rare-target raw-material sourcing, our one-stop platform supports your development from discovery to clinical application. Explore our capabilities in trans-sulfuration, redox biology, and hydrogen sulfide signaling to advance your next diagnostic or research project.