Anti-CYSLTR1 Rabbit Polyclonal Antibody for WB, ELISA - Q9Y271

Core Product Specifications and Parameters

Biological Background: CYSLTR1 Function and Localization

• CYSLTR1 is a G protein-coupled receptor for cysteinyl leukotrienes, with highest affinity for LTD4, followed by LTE4 and LTC4, and very low affinity for LTB4.
• Upon stimulation, it mediates bronchoconstriction and promotes smooth muscle contraction and proliferation, contributing to asthma pathophysiology.
• It triggers inflammatory responses including edema, eosinophil migration, and damage to the mucus layer in the lung.
• The receptor signals via a G protein that activates a phosphatidylinositol-calcium second messenger system.
• CYSLTR1 is localized to the cell membrane and belongs to the rhodopsin-like GPCR family.
• It is widely expressed, with highest levels in spleen and peripheral blood leukocytes, and lower expression in lung smooth muscle, alveolar macrophages, placenta, small intestine, pancreas, colon, and heart.
• Post-translational modifications include glycosylation and disulfide bond formation, typical of membrane receptors.

Experimental Guidance and Technical Tips

• For Western blot, a band around 39 kDa (theoretical 38.5 kDa) should be detected; glycosylation may cause additional bands or shifts.
• Verify the immunogen peptide sequence homology between human and mouse to ensure cross-reactivity; the antibody is listed as reactive with both species.
• For ELISA, use the antibody as a capture or detection reagent in a suitable sandwich or indirect format; titrate for optimal signal-to-noise ratio.
• Include appropriate positive and negative controls (e.g., tissues with known high expression like spleen or leukocytes) to confirm specificity.
• Consider the receptor’s cell membrane localization; use appropriate lysis conditions to extract membrane proteins efficiently.

CamelBio: Your One-Stop Sourcing Bridge

This antibody targets the cysteinyl leukotriene receptor 1, a key mediator in inflammatory and respiratory diseases such as asthma. At CamelBio, we support diagnostic manufacturers and research labs with one-stop access to validated IVD raw materials—from optimized polyclonal and monoclonal antibodies to bulk ancillary reagents and rare-target sourcing—helping you move from concept to clinic with reliable supply solutions.