Anti-CYPOR Rabbit Polyclonal Antibody for WB and ELISA - P16435

Core Product Specifications and Parameters

Biological Background: CYPOR Function and Localization

• CYPOR (NADPH--cytochrome P450 reductase), also known as CPR or P450R, is a 77 kDa flavoprotein encoded by the POR gene in humans.
• It functions as a key electron donor, transferring electrons from NADPH to cytochrome P450 enzymes in the endoplasmic reticulum, essential for microsomal monooxygenase reactions.
• Additionally, CYPOR supplies electrons to heme oxygenase and cytochrome B5, supporting diverse oxidative processes.
• The enzyme is anchored to the endoplasmic reticulum membrane via a transmembrane helix, with its catalytic domain facing the cytosol.
• Post-translational modifications include acetylation and phosphorylation; it binds FAD and FMN as cofactors.
• Mutations in POR are associated with disorders such as congenital adrenal hyperplasia and craniosynostosis, highlighting its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-250), which includes the transmembrane domain, likely generating antibodies against a conserved epitope enabling cross-reactivity with mouse and rat CYPOR.
• For Western blotting, a band around 77 kDa is expected in lysates from human, mouse, or rat tissues; validate specificity using knockout cell lines or siRNA knockdown.
• In ELISA, consider using the recombinant immunogen as a positive control; antibody affinity may be assessed with antigen titration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated partner for IVD raw materials, offering validated antibody pairs, bulk ancillary reagents, and custom sourcing solutions. For researchers investigating cytochrome P450-mediated drug metabolism or redox biology, CamelBio provides high-quality antibodies like this anti-CYPOR polyclonal antibody alongside technical support to streamline your assay development. We help bridge the gap from concept to clinic with reliable supply and expertise.