Anti-CYPOR Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P16435

Core Product Specifications and Parameters

Biological Background: CYPOR Function and Localization

• CYPOR (NADPH--cytochrome P450 reductase) is encoded by the POR gene. This flavoprotein contains FAD and FMN cofactors.
• Serves as the electron donor from NADPH to microsomal cytochrome P450 enzymes, pivotal in drug metabolism and steroidogenesis.
• Also transfers electrons to heme oxygenase and cytochrome B5.
• Localizes to the endoplasmic reticulum membrane via a transmembrane domain.
• Undergoes post-translational modifications including phosphorylation and acetylation.
• Clinically relevant mutations are associated with congenital adrenal hyperplasia and craniosynostosis.
• The solved 3D-structure aids in understanding its electron transfer mechanism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100-200 of human CYPOR, a region that is highly conserved; cross-reactivity with mouse and rat is expected but should be validated in the target sample system.
• For western blotting, a band of approximately 77 kDa is predicted; consider using reducing conditions and appropriate loading controls.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize fixation and antigen retrieval protocols to ensure detection of the membrane-bound protein.
• The antibody is suitable for ELISA applications; perform titration experiments to determine optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-CYPOR monoclonal antibody supports research in drug metabolism and steroidogenesis pathways. We offer validated antibody pairs, bulk reagents, and custom sourcing for rare targets to accelerate your assay development.