Anti-CYP7A1 Rabbit Polyclonal Antibody for IF-P and ELISA - P22680

Core Product Specifications and Parameters

Biological Background: CYP7A1 Function and Localization

• Cytochrome P450 7A1 (CYP7A1), also known as cholesterol 7-alpha-hydroxylase, is a monooxygenase that catalyzes the 7-alpha hydroxylation of cholesterol, the rate-limiting step in bile acid biosynthesis and cholesterol catabolism. Related references: PMID:12077124, PMID:19965590, PMID:2384150
• It also hydroxylates oxysterols such as 4beta-hydroxycholesterol and 24-hydroxycholesterol at the 7-alpha position. Related references: PMID:11013305, PMID:12077124
• CYP7A1 catalyzes the oxidation of the 7,8 double bond of 7-dehydrocholesterol and lathosterol, primarily forming 7-keto derivatives. Related references: PMID:21813643
• The enzyme is located in the endoplasmic reticulum and microsome membranes.
• It is predominantly expressed in the liver.
• As a heme-iron protein, CYP7A1 belongs to the cytochrome P450 family and plays essential roles in sterol and lipid metabolism.

Experimental Guidance and Technical Tips

• For immunofluorescence staining (IF-P), consider testing a range of antibody dilutions and include appropriate controls to confirm specificity for CYP7A1, which is primarily localized to the endoplasmic reticulum in human liver cells.
• When performing ELISA, validate the antibody pair compatibility to ensure optimal signal-to-noise ratios, particularly given the low abundance of CYP7A1 in many sample types.
• Since the immunogen corresponds to the N-terminal region (aa 1-100), the antibody may recognize full-length and potentially N-terminal fragments; confirm specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with streamlined access to high-quality IVD raw materials, including validated antibodies for cholesterol metabolism targets like CYP7A1. Our sourcing strength extends from optimized polyclonal antibodies to complete antibody pairs and bulk reagents, supporting your development from early-stage biomarker discovery to clinical testing. As your one-stop sourcing partner, CamelBio also specializes in delivering rare-target raw materials and tailored procurement solutions.