Anti-CYP51A1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q16850

Core Product Specifications and Parameters

Biological Background: CYP51A1 Function and Localization

• CYP51A1, also known as lanosterol 14-alpha demethylase, is a key enzyme in the cholesterol biosynthesis pathway. It converts lanosterol to 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol by removing the 14alpha-methyl group, a critical step for cholesterol production and subsequent steroid hormone synthesis. Related references: PMID:20149798 PMID:8619637 PMID:9559662
• The enzyme belongs to the cytochrome P450 superfamily (family 51, subfamily A) and functions as a monooxygenase, requiring heme and iron cofactors for its oxidative activity.
• CYP51A1 localizes to the endoplasmic reticulum membrane and microsomal fractions, consistent with its role in lipid metabolism at membrane sites.
• Alternative splicing of the CYP51A1 gene yields multiple isoforms, which may have distinct regulatory roles or tissue-specific expression patterns.
• Ubiquitously expressed, CYP51A1 shows highest levels in testis, ovary, adrenal, prostate, liver, kidney, and lung, reflecting the broad requirement for cholesterol in cellular membranes and hormone synthesis.
• The protein undergoes post-translational modifications such as ubiquitination (Ubl conjugation), which may regulate its stability or function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 245-420 of CYP51A1, a region that may be conserved across human, mouse, and rat, supporting the noted cross-reactivity.
• For Western blot (WB), the predicted molecular weight is approximately 57 kDa. Use appropriate positive control lysates from tissues with high expression such as testis or liver.
• When performing immunohistochemistry (IHC-P), antigen retrieval methods may be required, and staining should be validated in known positive tissue sections.
• For immunofluorescence (IF/ICC), consider permeabilization protocols to allow antibody access to the endoplasmic reticulum-localized target.
• ELISA applications may require optimization of coating antigen and detection system; validate with recombinant CYP51A1 protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support diagnostic development. This CYP51A1 antibody targets cholesterol metabolism, a pathway relevant to metabolic disorders and cancer research. Whether you need ready-to-use reagents or assistance in sourcing rare targets, CamelBio bridges the gap from concept to clinic.