Anti-CYP4V2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q6ZWL3

Core Product Specifications and Parameters

Biological Background: CYP4V2 Function and Localization

• CYP4V2 is a member of the cytochrome P450 superfamily, specifically belonging to the CYP4 family, which catalyzes the omega-hydroxylation of fatty acids.
• It functions as a monooxygenase in the endoplasmic reticulum membrane, utilizing heme iron as a cofactor to insert one oxygen atom into substrates using NADPH-derived electrons.
• CYP4V2 preferentially omega-hydroxylates polyunsaturated fatty acids (PUFAs) like docosahexaenoate (DHA) and eicosapentaenoate (EPA), playing a critical role in retinal PUFA homeostasis (PubMed:22772592). Related references: PMID:22772592
• It also metabolizes saturated fatty acids (laurate, myristate, palmitate) with varying efficiency, suggesting a broader role in lipid metabolism (PubMed:19661213). Related references: PMID:19661213
• The gene is widely expressed across tissues, with notable presence in retina and retinal pigment epithelium, consistent with its role in vision and corneal dystrophy pathologies.
• Keywords from UniProt highlight its involvement in vision, sensory transduction, and disease association with corneal dystrophy, as well as features like alternative splicing and transmembrane helices.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide within the central region of CYP4V2 (aa 200-300). For WB, the expected band size is approximately 61 kDa; consider using appropriate molecular weight markers and loading controls.
• For IF/ICC, optimize permeabilization and fixation protocols to detect endoplasmic reticulum membrane-bound CYP4V2; subcellular localization may vary with expression levels.
• ELISA applications may be used for detecting native or recombinant CYP4V2; validate assay conditions with known positive and negative controls.
• As a polyclonal antibody, batch-to-batch variability may occur; it is advisable to re-validate performance when switching to a new lot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering a wide range of antibodies, antigens, and ancillary reagents for diagnostic and research applications. This CYP4V2 polyclonal antibody is well-suited for studies on fatty acid metabolism and inherited retinal dystrophies, supporting researchers in ophthalmology and metabolic disease. CamelBio can further supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to streamline your assay development and large-scale procurement needs.