Anti-CYP4F12 Rabbit Polyclonal Antibody for WB and ELISA - Q9HCS2

Core Product Specifications and Parameters

Biological Background: CYP4F12 Function and Localization

• CYP4F12 encodes a cytochrome P450 monooxygenase of the CYP4 family, which primarily metabolizes fatty acids and xenobiotics.
• Catalyzes the hydroxylation of arachidonic acid to 18-hydroxy arachidonate with preference for the omega-2 position (PubMed:11162607). Related references: PMID:11162607
• Mediates epoxidation of double bonds in polyunsaturated fatty acids such as docosapentaenoic and docosahexaenoic acids (PubMed:16112640). Related references: PMID:16112640
• Shows low omega-hydroxylase activity toward leukotriene B4 and arachidonate (PubMed:11162645). Related references: PMID:11162645
• Involved in xenobiotic metabolism; hydroxylates the antihistamine drug ebastine (PubMed:11162645). Related references: PMID:11162645
• Localizes to the endoplasmic reticulum and microsomal membranes.
• Expressed in the small intestine, liver, colon, and heart.
• The protein contains a heme iron cofactor, features a transmembrane helix, and undergoes alternative splicing (UniProt keywords).

Experimental Guidance and Technical Tips

• The antibody is derived from rabbits immunized with a recombinant protein covering amino acids 265–524 of human CYP4F12. This region lies in the C-terminal half of the protein, which may contribute to specificity.
• Recommended for Western blotting (WB) and ELISA applications. Optimize dilution factors empirically using positive control lysates from liver or small intestine, which express CYP4F12.
• Predicted molecular weight is approximately 60 kDa; confirm using appropriate molecular weight markers.
• Cross-reactivity is reported for human, mouse, and rat samples. Validate detection in each species individually with appropriate controls.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical experiments. Consider using the same lot for longitudinal studies.
• For ELISA, pair with a suitable detection antibody; assess potential cross-reactivity with related CYP4F isoforms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For cytochrome P450 research and drug metabolism studies, we support sourcing of rare-target antibodies and custom development. Contact us to streamline your procurement from concept to clinic.