Anti-CYP46A1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y6A2

Core Product Specifications and Parameters

Biological Background: CYP46A1 Function and Localization

• CYP46A1 is a cytochrome P450 monooxygenase that functions as a cholesterol 24-hydroxylase, playing a major role in brain cholesterol homeostasis by catalyzing the hydroxylation at C-24 of the cholesterol side chain, thereby promoting cholesterol elimination from neurons. Related references: PMID:10377398 PMID:14640697 PMID:18621681
• In addition to cholesterol, it hydroxylates various cholesterol derivatives and steroid hormones on both the ring and side chain, converting them into active oxysterols that participate in lipid signaling and biosynthesis. Related references: PMID:12077124 PMID:14640697 PMID:28190002
• Acts as an epoxidase by converting desmosterol into (24S),25-epoxycholesterol, a ligand for nuclear receptors NR1H2 and NR1H3, which has been shown to promote neurogenesis in the developing brain. Related references: PMID:25017465
• May also catalyze the oxidative metabolism of xenobiotics such as clotrimazole. Related references: PMID:20667828
• By driving cholesterol elimination, CYP46A1 may indirectly activate the mevalonate pathway and coordinate the synthesis of new cholesterol and nonsterol isoprenoids involved in synaptic activity and learning (By similarity).
• Localizes to the endoplasmic reticulum membrane, microsome membrane, pre- and postsynaptic regions, and dendritic projections.
• Expression is brain‐specific, with higher mRNA levels in gray matter zones; expression increases linearly with age and is absent in fetal tissue.
• Keywords: Heme, iron, metal-binding, monooxygenase, oxidoreductase, sterol metabolism, cholesterol metabolism, synapse, transmembrane, alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 24–280, covering a large N‑terminal portion of the protein; while this region is likely to generate antibodies against full‑length CYP46A1, it is advisable to validate reactivity in the relevant sample system, especially when detecting potential isoforms or fragments.
• For Western blotting, a band near 57 kDa is expected; consider using a 10% SDS‑PAGE gel and appropriate transfer conditions for this molecular weight range.
• In immunofluorescence/ICC, since CYP46A1 is an integral membrane protein of the ER, mild permeabilization with detergents such as 0.1% Triton X‑100 is recommended; co‑staining with an ER marker may confirm localization.
• For ELISA applications, titrate the antibody as a capture or detection reagent and optimize using a known positive control (e.g., recombinant CYP46A1 protein or tissue lysate); cross‑reactivity with mouse and rat orthologs supports use in rodent models.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting—supporting every stage from concept to clinic. For CYP46A1‑related research and assay development, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare‑target raw materials to streamline your project workflow.