Anti-CYP46A1 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - Q9Y6A2

Core Product Specifications and Parameters

Biological Background: CYP46A1 Function and Localization

• CYP46A1 (Cholesterol 24-hydroxylase, also known as CP46, CYP46) is a member of the cytochrome P450 superfamily, functioning as a heme-thiolate monooxygenase.
• It primarily catalyzes the 24S-hydroxylation of cholesterol in the brain, a key step for cholesterol elimination from neurons and overall brain cholesterol homeostasis (PubMed:10377398, PubMed:14640697, PubMed:18621681). Related references: PMID:10377398 PMID:14640697 PMID:18621681
• CYP46A1 also hydroxylates cholesterol derivatives and hormone steroids, generating oxysterols that participate in lipid signaling and biosynthesis (PubMed:12077124, PubMed:14640697, PubMed:28190002). Related references: PMID:12077124 PMID:14640697 PMID:28190002
• It acts as an epoxidase, converting desmosterol into (24S),25-epoxycholesterol, an oxysterol that promotes neurogenesis via nuclear receptor activation (PubMed:25017465). Related references: PMID:25017465
• The enzyme is localized to the endoplasmic reticulum membrane, microsomes, and also postsynaptic and presynaptic regions of neurons, including dendritic projections.
• In brain, CYP46A1 expression is higher in gray matter and increases linearly with age; it is not detected in fetal tissue.
• CYP46A1 may also metabolize xenobiotics such as clotrimazole, indicating a potential role in drug metabolism in the brain (PubMed:20667828). Related references: PMID:20667828

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to residues 400–500 of human CYP46A1. This region may be surface-exposed; consider potential interference from protein–protein interactions or post-translational modifications near this epitope when interpreting results.
• For Western blot (WB), the predicted molecular weight is 57 kDa. Validate band specificity by comparing with a negative control or using a blocking peptide if available.
• For immunofluorescence on paraffin-embedded sections (IF-P), optimize antigen retrieval, blocking, and antibody dilution according to your sample type. A nuclear counterstain is recommended to differentiate cytoplasmic/membrane staining.
• For ELISA, test the antibody as both capture and detection reagent in a checkerboard titration to determine optimal concentrations. Pair with a validated matched antibody for sandwich applications after in-house validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with a comprehensive portfolio of IVD raw materials. This anti-CYP46A1 polyclonal antibody, targeting a key enzyme in brain cholesterol metabolism, exemplifies our commitment to providing high-quality reagents for neuroscience and lipid disorder research. Beyond individual antibodies, we supply validated antibody pairs, bulk ancillary reagents, and custom sourcing services to accelerate your assay development from concept to clinic.