Anti-CYP3A5 Polyclonal Antibody for WB, ELISA - P20815

Core Product Specifications and Parameters

Biological Background: CYP3A5 Function and Localization

• CYP3A5, also known as CYPIIIA5 or cytochrome P450-PCN3, is a member of the cytochrome P450 superfamily of monooxygenases, which catalyze the insertion of one oxygen atom into organic substrates using NADPH and molecular oxygen. Related references: PMID:10681376, PMID:11093772, PMID:12865317
• It plays a key role in the metabolism of steroid hormones, including the hydroxylation of testosterone, progesterone, and androstenedione at the 6β-position, and the conversion of 17β-estradiol and estrone to catechol estrogens. Related references: PMID:2732228, PMID:12865317
• CYP3A5 is involved in vitamin A metabolism, catalyzing the oxidation of all-trans-retinol to all-trans-retinal, a rate-limiting step in retinoic acid biosynthesis, and further metabolizes retinoic acid to 4-hydroxyretinoate. Related references: PMID:10681376, PMID:11093772
• It also metabolizes xenobiotic compounds, such as the immunosuppressant cyclosporine and the calcium channel blocker nifedipine, contributing to drug clearance and pharmacokinetic variability. Related references: PMID:2732228
• The protein localizes to the endoplasmic reticulum membrane and microsomal fraction, consistent with its role in oxidative metabolism.
• CYP3A5 is subject to alternative splicing, producing multiple transcript variants, and its expression is highly polymorphic, influencing interindividual differences in drug response.
• It is a heme‑ and iron‑containing enzyme, classified under oxidoreductases, with a molecular weight of approximately 57.1 kDa.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a recombinant fragment (amino acids 233–502) of human CYP3A5; this region may be suitable for detecting several isoforms, but cross‑reactivity with other CYP3A family members (e.g., CYP3A4, CYP3A7) should be evaluated when interpreting results.
• For Western blot (WB), a band around 57 kDa is expected; verify detection in human, mouse, or rat tissue or cell lysates known to express CYP3A5 (e.g., liver, intestine).
• In ELISA applications, consider pairing this polyclonal antibody as a capture or detection reagent; performance should be validated using recombinant CYP3A5 protein or native samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material procurement, providing validated antibody pairs, optimized recombinant proteins, and ancillary reagents to streamline diagnostic assay development. For researchers working on drug metabolism and pharmacogenomics, we offer reliable access to CYP3A5 antibodies and related panel components. Whether you need bulk quantities, custom formulations, or rare target sourcing, CamelBio bridges the gap from concept to clinic to accelerate your IVD projects.