Anti-CYP3A4 Rabbit Polyclonal Antibody for WB and ELISA - P08684

Core Product Specifications and Parameters

Biological Background: CYP3A4 Function and Localization

• CYP3A4 is a cytochrome P450 monooxygenase that catalyzes the NADPH-dependent insertion of molecular oxygen into a wide range of substrates, including sterols, steroid hormones, retinoids, and fatty acids (PubMed:10681376, PubMed:11093772, PubMed:11555828, PubMed:12865317, PubMed:14559847, PubMed:15373842). Related references: PMID:10681376, PMID:11093772, PMID:11555828, PMID:12865317, PMID:14559847, PMID:15373842
• It plays a major role in the oxidative metabolism of xenobiotics, including many clinical drugs such as nifedipine, albendazole, and quinine (PubMed:8968357, PubMed:10759686, PubMed:11159812, PubMed:11695850). Related references: PMID:8968357, PMID:10759686, PMID:11159812, PMID:11695850
• CYP3A4 is critical for steroid hormone homeostasis, converting testosterone to less active hydroxylated metabolites and generating hydroxyestrogens from estrone and estradiol (PubMed:11555828, PubMed:12865317, PubMed:14559847, PubMed:15373842, PubMed:15764715, PubMed:22773874, PubMed:2732228). Related references: PMID:11555828, PMID:12865317, PMID:14559847, PMID:15373842, PMID:15764715, PMID:22773874, PMID:2732228
• The enzyme contributes to cholesterol degradation and bile acid biosynthesis by hydroxylating cholesterol at the C-4β and C-25 positions (PubMed:21576599). Related references: PMID:21576599
• CYP3A4 metabolizes polyunsaturated fatty acids (PUFAs) via bisallylic hydroxylation and epoxidation, and converts endocannabinoids into epoxyeicosatrienoic acid ethanolamides, potentially modulating signaling (PubMed:9435160, PubMed:19965576, PubMed:20702771). Related references: PMID:9435160, PMID:19965576, PMID:20702771
• It is involved in retinoid metabolism, oxidizing all-trans-retinol to all-trans-retinal and further metabolizing all-trans-retinoic acid (PubMed:10681376, PubMed:11093772). Related references: PMID:10681376, PMID:11093772
• CYP3A4 is anchored to the endoplasmic reticulum and microsomal membranes via an N-terminal transmembrane helix.
• Tissue expression is highest in liver and prostate, with additional detection in small intestine, kidney, adrenal cortex, and other tissues (PubMed:19094056, PubMed:18545703, PubMed:19359404). Related references: PMID:19094056, PMID:18545703, PMID:19359404

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 244–503) of human CYP3A4, which may influence epitope accessibility and cross-reactivity profiles; consider this when designing detection assays.
• For Western blotting, a band at approximately 57 kDa is expected; validate with appropriate positive controls (e.g., human liver microsomes or recombinant protein) and verify specificity in your sample system.
• In ELISA applications, optimize coating and blocking conditions using purified antigen or standard reference preparations.
• As a polyclonal antibody, batch performance may vary; always confirm reactivity with your target species and model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers and research teams working on CYP3A4-driven drug metabolism, pharmacokinetics, and steroid biology. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare target raw materials. Our integrated supply chain support helps bridge the gap from early research concepts to clinical development, keeping your projects efficient and on track.