Anti-CYP3A4 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P08684

Core Product Specifications and Parameters

Biological Background: CYP3A4 Function and Localization

• Cytochrome P450 3A4 (CYP3A4) is a heme-thiolate monooxygenase belonging to the cytochrome P450 superfamily, playing a pivotal role in the oxidative metabolism of a wide range of endogenous compounds and xenobiotics.
• Major drug-metabolizing enzyme: Catalyzes sulfoxidation of albendazole and fenbendazole, and hydroxylation of quinine, making it a key determinant of drug efficacy and toxicity (PubMed:10759686, PubMed:8968357). Related references: PMID:10759686, PMID:8968357
• Steroid hormone metabolism: Converts testosterone to less-active 2beta- and 6beta-hydroxytestosterones and forms hydroxyestrogens from estrone and 17beta-estradiol, regulating hormone activity (PubMed:15373842, PubMed:15764715, PubMed:2732228, PubMed:11555828, PubMed:12865317, PubMed:14559847). Related references: PMID:15373842, PMID:15764715, PMID:2732228, PMID:11555828, PMID:12865317, PMID:14559847
• Cholesterol degradation and bile acid biosynthesis: Contributes to the formation of 4beta- and 25-hydroxycholesterols, involved in cholesterol homeostasis (PubMed:21576599). Related references: PMID:21576599
• Retinoid metabolism: Oxidizes all-trans-retinol to all-trans-retinal and further metabolizes retinoic acid, influencing signaling pathways (PubMed:10681376, PubMed:11093772). Related references: PMID:10681376, PMID:11093772
• Lipid and endocannabinoid metabolism: Catalyzes bisallylic hydroxylation and epoxidation of polyunsaturated fatty acids, and oxidizes anandamide, potentially modulating lipid signaling (PubMed:9435160, PubMed:19965576, PubMed:20702771). Related references: PMID:9435160, PMID:19965576, PMID:20702771
• Subcellular localization: CYP3A4 is anchored to the endoplasmic reticulum membrane, where it functions in drug and lipid metabolism.
• Tissue expression: Highly expressed in liver and intestinal epithelia, with lower levels in prostate, kidney, and other tissues, consistent with its roles in systemic clearance and extrahepatic metabolism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 244-503 of human CYP3A4, a region that may include epitopes conserved among mammals; cross-reactivity with mouse and rat is reported, but verification in the model system is advised.
• For Western blotting, detect a band at approximately 57 kDa. Use RIPA buffer with adequate detergents to solubilize membrane-bound CYP3A4.
• Liver and intestinal tissue lysates serve as strong positive controls due to high endogenous expression.
• For IHC-P and IF/ICC, perform antigen retrieval and include appropriate isotype controls to confirm staining specificity.
• ELISA applications may require titration of both capture and detection antibodies; optimize conditions for your plate format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive raw-material solutions for IVD development, including validated antibody pairs, bulk reagents, and custom sourcing for specialized targets. This anti-CYP3A4 product supports research into drug metabolism, toxicology, and endocrine function, complemented by our extensive catalog of ancillary reagents and technical support. Let us accelerate your assay development from concept to clinic.