Anti-CYP39A1 Rabbit Polyclonal Antibody for WB and ELISA - Q9NYL5

Core Product Specifications and Parameters

Biological Background: CYP39A1 Function and Localization

• CYP39A1 is a cytochrome P450 monooxygenase, also known as 24-hydroxycholesterol 7-alpha-hydroxylase or oxysterol 7-alpha-hydroxylase, encoded by the CYP39A1 gene in humans.
• It catalyzes the 7-alpha hydroxylation of (24S)-hydroxycholesterol, a key step in the conversion of neural oxysterols to bile acids in the liver, thereby contributing to cholesterol clearance from the brain. Related references: PMID:10748047 PMID:25201972
• The enzyme localizes to the endoplasmic reticulum membrane and microsome membrane, consistent with its role in hepatic metabolism.
• Expression of CYP39A1 is liver-specific, highlighting its specialized function in hepatic bile acid synthesis.
• CYP39A1 belongs to the cytochrome P450 superfamily, characterized by a heme iron active site, and functions as a monooxygenase/oxidoreductase, requiring NADPH and cytochrome P450 reductase for electron transfer.
• Additional keywords associated with CYP39A1 include cholesterol metabolism, steroid/sterol metabolism, transmembrane helix, and signal peptide, reflecting its enzymatic and structural features.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100) of human CYP39A1. This antibody may recognize the native N-terminal epitope; consider validating in denaturing versus native conditions for applications like ELISA.
• For Western blot (WB), the predicted molecular weight is 54 kDa; ensure appropriate gel percentage and transfer conditions for this size protein.
• Cross-reactivity with mouse and rat is reported; confirm expression in relevant tissue lysates (e.g., liver) as the protein is liver-specific.
• When performing ELISA, optimize coating antigen and antibody concentrations; the immunogen peptide may serve as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions streamlined access to high-quality IVD raw materials, including this anti-CYP39A1 polyclonal antibody. As a one-stop sourcing partner, we support projects in cholesterol metabolism and liver-related biomarker research with validated antibody pairs, optimized monoclonals, and bulk ancillary reagents to accelerate your development from concept to clinic.