Anti-CYP39A1 Rabbit Polyclonal Antibody for WB and ELISA - Q9NYL5

Core Product Specifications and Parameters

Biological Background: CYP39A1 Function and Localization

• CYP39A1, also known as 24-hydroxycholesterol 7-alpha-hydroxylase or oxysterol 7-alpha-hydroxylase, is a member of the cytochrome P450 monooxygenase superfamily.
• It catalyzes the 7-alpha hydroxylation of (24S)-hydroxycholesterol, a critical step in the conversion of neural oxysterols to bile acids, facilitating cholesterol clearance from the brain. Related references: PMID:10748047 PMID:25201972
• The enzyme localizes to the endoplasmic reticulum and microsomal membranes, functioning as an integral membrane protein.
• Expression is liver-specific, consistent with its role in hepatic metabolism of oxysterols derived from the brain.
• CYP39A1 is a heme- and iron-containing monooxygenase that requires molecular oxygen and NADPH-cytochrome P450 reductase for its catalytic activity.
• It belongs to the cytochrome P450 family 39, subfamily A, polypeptide 1, and is involved in cholesterol and steroid metabolic pathways.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal region (aa 300-400) of human CYP39A1, which shares sequence homology with mouse and rat orthologs, underpinning the observed cross-reactivity.
• For Western blot detection, consider using human liver microsomal extract as a positive control due to the liver-specific expression of the target.
• As a polyclonal antibody, validate specificity in your experimental system, as off-target binding may occur; pre-adsorption or comparison with knockout controls is recommended.
• The predicted molecular weight is ~54 kDa; however, post-translational modifications or sample preparation may cause slight mobility shifts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing platform for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For cholesterol metabolism and liver function research, we can assist in sourcing antibodies against rare targets like CYP39A1 and offer customized immunoreagent development to support your assay and diagnostic pipeline. Contact us to streamline your procurement and accelerate development.