Anti-CYP39A1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9NYL5

Core Product Specifications and Parameters

Biological Background: CYP39A1 Function and Localization

• CYP39A1 encodes a cytochrome P450 monooxygenase known as 24-hydroxycholesterol 7-alpha-hydroxylase, also called oxysterol 7-alpha-hydroxylase.
• It catalyzes the 7-alpha hydroxylation of (24S)-hydroxycholesterol, a key step in converting neural oxysterols into bile acids in the liver, thus contributing to neural cholesterol clearance. Related references: PMID:10748047 PMID:25201972
• The enzyme uses molecular oxygen and NADPH via cytochrome P450 reductase to insert one oxygen atom into the substrate and reduce the second to water. Related references: PMID:10748047 PMID:25201972
• CYP39A1 is a member of the cytochrome P450 superfamily and features a heme-iron active site, characteristic of its monooxygenase and oxidoreductase functions.
• Subcellularly, it localizes to the endoplasmic reticulum membrane and microsome membrane.
• Tissue expression is liver-specific, consistent with its role in hepatic bile acid synthesis.
• The protein contains a transmembrane helix and a signal sequence, with a predicted molecular weight of approximately 54 kDa (UniProt: 54116 Da).

Experimental Guidance and Technical Tips

• For Western blotting, consider a starting dilution range of 1:500–1:2000, and validate with appropriate positive and negative controls (e.g., liver lysates).
• In IHC-P, antigen retrieval using heat-induced epitope retrieval (HIER) at pH 6 or 9 may enhance detection; titrate the antibody to optimize staining intensity.
• ELISA performance can be optimized by direct or sandwich formats, pairing with suitable recombinant protein or peptide antigens.
• Since the immunogen is a peptide from amino acids 301–400, the antibody may cross-react with mouse CYP39A1 due to sequence homology; confirm reactivity in your experimental system.
• Given liver-specific expression, liver tissue lysates or sections are recommended as positive controls; use knockout models or siRNA to verify specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers focusing on cholesterol metabolism or liver-related pathways, we provide one-stop access to high-quality primary antibodies like anti-CYP39A1, ensuring reliable supply for assay development. Our team also supports rare target raw-material sourcing to accelerate your diagnostic projects from concept to clinic.