Anti-CYP2R1 Rabbit Polyclonal Antibody for WB, ELISA - Q6VVX0

Core Product Specifications and Parameters

Biological Background: CYP2R1 Function and Localization

• CYP2R1 encodes vitamin D 25-hydroxylase, a cytochrome P450 monooxygenase that catalyzes hydroxylation at C-25 of both vitamin D2 and D3, a critical activation step in vitamin D metabolism (PubMed:12867411, PubMed:15465040, PubMed:18511070).

Related references: PMID:12867411 PMID:15465040 PMID:18511070

• It also metabolizes the prodrugs 1alpha-hydroxyvitamin D2 (doxercalciferol) and 1alpha-hydroxyvitamin D3 (alfacalcidol) into their active 25-hydroxylated forms (PubMed:15465040, PubMed:18511070).

Related references: PMID:15465040 PMID:18511070

• The reaction uses molecular oxygen and electrons donated by NADPH via cytochrome P450 reductase, inserting one oxygen atom into the substrate (PubMed:12867411, PubMed:15465040, PubMed:18511070).

Related references: PMID:12867411 PMID:15465040 PMID:18511070

• CYP2R1 localizes to the endoplasmic reticulum and microsome membranes, characteristic of membrane-bound P450 enzymes involved in lipid-soluble substrate metabolism.

• The protein is classified under lipid metabolism, heme and iron binding, and monooxygenase activity. Several disease-associated variants have been reported, and a three-dimensional structure is available.

Experimental Guidance and Technical Tips

• For Western blot, anticipate a band around 57 kDa (predicted molecular weight 57.3 kDa). Validate detection in lysates from liver or other vitamin D-metabolizing tissues.
• The immunogen targets the C-terminal region (aa 252–501), so the polyclonal antibody may recognize full-length CYP2R1 and potential processed forms.
• This antibody cross-reacts with mouse and rat, enabling studies across these species.
• For ELISA applications, consider pairing with a detection antibody against a different epitope to achieve optimal sensitivity.
• Since the antibody is provided as a polyclonal, batch-to-batch consistency should be verified for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material procurement, offering validated antibody pairs, optimized mono- and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing to support vitamin D metabolism research and clinical assay development. Through our global supplier network, we streamline the transition from discovery to diagnostic application, ensuring reliable access to essential reagents like anti-CYP2R1 for your next project.