Anti-CYP2F1 Polyclonal Antibody for WB, IHC-P, ELISA - P24903

Core Product Specifications and Parameters

Biological Background: CYP2F1 Function and Localization

• Cytochrome P450 2F1 (CYP2F1) is a member of the cytochrome P450 superfamily of monooxygenases, which catalyze the oxidation of organic substances and are key in the metabolism of xenobiotics and endogenous compounds.
• The enzyme may participate in the metabolism of various pneumotoxicants, including naphthalene, and is capable of dealkylating ethoxycoumarin, propoxycoumarin, and pentoxyresorufin, though it shows no activity toward ethoxyresorufin and only trace dearylation activity toward benzyloxyresorufin.
• CYP2F1 bioactivates 3-methylindole (3MI) via dehydrogenation to the putative electrophile 3-methylene-indolenine, a compound implicated in pulmonary toxicity.
• This protein localizes to the endoplasmic reticulum membrane and microsome membrane, consistent with its role in the microsomal monooxygenase system.
• Tissue expression is predominantly in the lung, with rare detection in liver and placenta, highlighting its specialized function in respiratory tract metabolism.
• CYP2F1 contains a heme prosthetic group and iron cofactor, essential for its catalytic activity, and is classified as an oxidoreductase. Alternative splicing of the CYP2F1 gene has been reported, potentially yielding different isoforms.
• The calculated molecular weight of the canonical isoform is approximately 55.5 kDa, consistent with the observed band at 56 kDa on SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100–290 of human CYP2F1, a region likely within the cytoplasmic domain that may exhibit sufficient conservation across species to allow the observed human, mouse, and rat cross-reactivity.
• For Western blot, a typical starting dilution for polyclonal antibodies can be explored (e.g., 1:500–1:2000), and the assay should be validated in a lung-derived cell line or tissue lysate where CYP2F1 expression is expected.
• In IHC-P, antigen retrieval methods (e.g., heat-induced epitope retrieval at pH 6 or 9) may be necessary; include appropriate controls such as lung tissue sections. Preliminary testing with different fixation protocols is recommended.
• For ELISA, the antibody can be used as a capture or detection reagent in a sandwich format, but cross-reactivity with other CYP family members should be assessed due to sequence similarities.
• Since CYP2F1 is a membrane protein, consider using mild detergents in lysis buffers (e.g., 1% NP-40 or Triton X-100) to ensure proper solubilization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, streamlining procurement for diagnostic manufacturers. With expertise in cytochrome P450 targets, CamelBio can support lung toxicology and respiratory disease research by supplying the necessary high-quality raw materials. For rare or customized targets like CYP2F1, CamelBio provides one-stop sourcing and technical consulting to bridge the gap from concept to clinical application.