Anti-CYP2E1 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P05181

Core Product Specifications and Parameters

Biological Background: CYP2E1 Function and Localization

• CYP2E1 is a cytochrome P450 monooxygenase that plays a central role in the metabolism of fatty acids, specifically hydroxylating saturated fatty acids at the omega-1 position with high catalytic activity.
  Related references: PMID:10553002 PMID:18577768
• The enzyme is localized to the endoplasmic reticulum membrane, microsome membrane, and mitochondrial inner membrane, facilitating its role in lipid metabolism and xenobiotic processing.
• CYP2E1 is also known by aliases including 4-nitrophenol 2-hydroxylase, CYPIIE1, and Cytochrome P450-J, reflecting its substrate specificity and gene family.
• It is a heme-thiolate protein requiring NADPH as a cofactor, with iron in the heme group essential for its monooxygenase activity.
• Beyond fatty acid metabolism, CYP2E1 is likely involved in the oxidative metabolism of various xenobiotics, contributing to detoxification and bioactivation processes.
• The protein has a molecular weight of approximately 57 kDa, consistent with its predicted mass and the product's observed band.

Experimental Guidance and Technical Tips

• Since the antibody was raised against a recombinant fragment spanning amino acids 234–493, which includes the catalytic domain, researchers may consider this region for immunogen design when developing antibodies for similar targets.
• For Western blot, the predicted molecular weight is 57 kDa; validate detection in relevant lysates such as human, mouse, or rat liver tissue, where CYP2E1 is highly expressed.
• When performing immunohistochemistry or immunofluorescence, optimize antigen retrieval and antibody dilution, as polyclonal antibodies may require empirical titration for each application.
• For ELISA applications, consider pairing with a suitable capture antibody for sandwich ELISA development; the polyclonal nature may provide broad epitope recognition.

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CamelBio offers diagnostic manufacturers and research laboratories a streamlined, one-stop sourcing platform for high-quality IVD raw materials. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies like this anti-CYP2E1, and bulk ancillary reagents to support drug metabolism and toxicology assay development. Whether you require established catalog products or custom rare-target raw-material sourcing, CamelBio bridges the gap from concept to clinic with end-to-end supply solutions.